Scientific Posters
Scientists from Cell Signaling Technology regularly present posters at scientific conferences. Our studies involve the development of new technologies for signaling pathway analysis and mechanistic cell biology research.
Portable Document Format (PDF) files of our investigative research are linked below. Some of the PDFs are large files. The sizes for each file are listed.
2012
- Comprehensive Quantitative Profiling of the Mouse Kinome using Data-independent LC-MS-MS/MSALL with SWATH™ Acquisition.
- Jeffrey C. Silva, Matthew P. Stokes, Charles L. Farnsworth, Xiaoying Jia, Christie Hunter
909 KB PDF - Quantitative Profiling of Signaling Pathways Using PTMScan® Direct.
- Matthew P. Stokes, Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Roberto Polakiewicz, Michael J Comb
2.2 MB PDF - The Large-scale Analysis of Protein Expression in Human Ovarian Tumor Samples by LC-MS/MS/MS.
- Anthony Possemato, Sean Beausoleil, Klarisa Rikova, Hong Ren, Kimberly Lee, Michael Comb
1.7 MB PDF - Multiplexing Kinase Substrate Motif Antibodies for Expanded Coverage of the Serine/Threonine Kinome.
- Charles L. Farnsworth, Jeffrey C. Silva, Matthew P. Stokes, Hongbo Gu, Ailan Guo, Kimberly A. Lee, Jian-Min Ren, Xiaoying Jia, Roberto D. Polakiewicz, Michael J. Comb
717 KB PDF - Immunoaffinity Enrichment and Quantitative Profiling of Protein Phosphorylation, Methylation, and Acetylation in Receptor Tyrosine Kinase Pathway-addicted Cancer Cell Lines.
- Klarisa Rikova, Ailan Guo, Kimberly A. Lee, Anthony Possemato, Yi Wang, Sean A. Beausoleil, Daniel E. Mulhern, Jian Min Ren, Michael J. Comb
2.5 MB PDF - A Proteomics Approach for the Identification and Cloning of Monoclonal Antibodies from Serum.
- Wan Cheung (Gabriel) Cheung*, Sean A. Beausoleil*, Xiaowu Zhang, Shuji Sato, Sandra M. Schieferl, James S. Wieler, Jason G. Beaudet, Ravi K. Ramenani, Lana Popova, Michael J. Comb, John Rush, and Roberto D. Polakiewicz.
*These authors contributed equally to this work
1.4 MB PDF - Over-expression of EphB1 leads to Stat3 phosphorylation.
- Danielle Swain, Richard Noring, Jake Knowles, Roberto Campos-González, Zev Gechtman
1.1 MB PDF - Rapid quantification of autophagy in cell-based assays.
- Christopher A. Manning, Michael R. Nelson, Paul Wylie, Diana Caracino, Randall K. Wetzel
1.9 MB PDF
2011
- The use of a four-color multiplex antibody-based assay with laser-scanning high content screening to examine kinase inhibitor effects on cellular signaling.
- Jessica A. Cherry, Paul Wylie, Christopher A. Manning, Diana Caracino, Randall K. Wetzel
596 KB PDF - PTMScan® Direct: Quantitative Profiling of Critical Nodes From Cellular Signaling Pathways.
- Matthew P. Stokes, Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Albrecht Moritz, Ailan Guo, John Rush, Roberto Polakiewicz, Michael J. Comb
8.1 MB PDF
2010
- IHC with Mutation-Specific Antibodies Detecting the Status of EGFR Mutations in NSCLC.
- Jian Y, Daiqiang Li, Chunhong Hu
770 KB PDF - Frequencies of ALK and ROS in NSCLC FFPE tumor samples utilizing a highly specific and sensitive immunohistochemistry-based assay and FISH analysis.
- V. Rimkunas, K. Crosby, M. Silver, K. Hincman, M. Kelly, D. Li, X.Zhou, R.H. Haack
885 KB PDF
2008
- Mutation-Specific Antibodies for the Detection of EGFR Mutations in Non-Small-Cell Lung Cancer.
- Jian Yu, Susan Kane, Daiqiang Li, Herbert Haack, Bradley Smith, Ting-Lei Gu, Massimo Loda, Xinmin Zhou, Michael J. Comb
1.6 MB PDF - Analysis of kinase inhibitors using a large array of signaling antibodies and high content screening.
- Jessica A. Cherry, Corinne Michaud, Paul Wylie, Randall K. Wetzel
852 KB PDF
2007
- Evaluation of PTEN: Immunohistochemical Analysis of Tissues and Cells.
- Angela Lightbown, Qingfu Zeng, Michael Lewis, Katherine Crosby, Bradley L. Smith
360 KB PDF - Detection of Genetic Abnormalities in Cancer Cells using Bio-Plex® Immuno-Assays.
- Andrew Romano, Kathleen Rogers, Susan Kane, Jeff Lee, Whei Feng, Bradley L. Smith, Reggie Prioli
246 KB PDF - Phosphorylation Signatures Identify Tyrosine Kinases Activated in Lung Cancer.
- Klarisa Rikova, Ailan Guo, Qingfu Zeng, Anthony Possemato, Herbert Haack, Julie Nardone, Kimberly Lee, Yu Li, Laura Sullivan, Joan MacNeill, Corey Bakalarski, Judit Villen, John Kornhauser, John Rush, Steven Gygi, Roberto Polakiewicz, Michael Comb
822 KB PDF - Rapid and Quantitative Cell-Based Immunoaffinity Kinase Assay in a 96-well Format.
- Jin Yuan, Craig Thompson
569 KB PDF - The Detection of Phosphoproteins by Bio-Plex® Assays in Cell, Xenograft and Human Tumor Lysate.
- Jeremy Fisher, Kathleen Rogers, Katherine Crosby, Andrew Romano, Bradley L. Smith, Reginaldo Prioli
512 KB PDF - PhosphoSite®: A Knowledge Base of Post-Translational Modifications and Their Role in Cell Signaling.
- David Merberg, Roland Carel, Jon Kornhauser, Juan Munoz, Beth Murray, Julie Nardone, Ousama Shamma, Elzbieta Skrzypek, Bin Zhang, Peter Hornbeck
1.1 MB PDF - Phospho-Specific Rabbit Monoclonal Antibodies Validated for Immunohistochemistry.
- Cynthia Shapiro, Angela Lightbown, Kathleen Rogers, Jessica Simendinger, Michael Lewis, Katherine Crosby
2.9 MB PDF
2006
- Expression of Active Tyrosine Kinases Using a Baculovirus Expression System: Tools for Drug Discovery and Cell Signaling Studies.
- Ann Liu, Jing Li, Jin Yuan, Bevin Philip, Craig Thompson
344 KB PDF - Analysis of Flt3 cellular signaling in leukemic cell lines by flow cytometry and phospho-specific antibodies.
- Lana Popova, Brett Norris, Ting-Lei Gu, Randall K. Wetzel, Bradley L. Smith, Roberto Polakiewicz
426 KB PDF - Comparison of Immunohistochemical Staining of Signaling Markers in Frozen and Paraffi n-Embedded Tissues.
- Katherine Crosby, Cynthia Shapiro, Michael Lewis, Angela Lightbown, Qingfu Zeng, Bradley L. Smith
430 KB PDF - Utility of evolutionary conservation in ranking phosphorylation sites discovered by high-throughput methods.
- Julie Nardone, Yi Wang, Bin Zhang, Jon Kornhauser, Elzbieta Skrzypek, Vicky Yang, David Merberg, Roberto Polakiewicz, Peter Hornbeck
168 KB PDF - Large Scale Profiling of Phospho-proteins in Cell and Tissue Microarrays using Phospho-Specific Antibodies: Identification of Biomarkers and Pharmacodynamic activity for a c-Kit inhibitor.
- Herbert Haack, Laura C. Sullivan, Andrew Garton, John D. Haley, Bradley L. Smith
254 KB PDF - Optimization and Validation of Activation-State Specific Antibodies for the Immunohistochemical Analysis of Fresh Frozen Tissues and Cells.
- Cynthia Shapiro, Katherine Crosby, Michael Lewis, Bradley L. Smith
639 KB PDF - Comparison of Protein Phosphorylation in Cell Line and Xenograft Samples by Bio-Plex® and Western Blot Techniques.
- Kathleen Rogers, Jeremy Fisher, Michael Lewis, Bradley L. Smith, Reginaldo Prioli
250 KB PDF - Detection of Genetic Abnormalities in Cancer Cells using Bio-Plex® Immuno-Assays.
- Andrew Romano, Kathleen Rogers, Susan Kane, Jeff Lee, Whei Feng, Bradley L. Smith, Reggie Prioli
209 KB PDF - Immunohistochemical Analysis of Potential Cell Signaling Biomarkers for Mutant Epidermal Growth Factor Receptor.
- Qingfu Zeng, Katie Crosby, Ailan Guo, Klarisa Rikova, Bradley L. Smith, Roberto Polakiewicz
242 KB PDF
2005
- Development and Use of Phosphorylation-Specific Capture and Detection Antibodies.
- Michael D. Melnick, Bradley L. Smith, Christopher Bunker
377 KB PDF - Evaluation of PTEN: Immunohistochemical Analysis of Tissues and Cells.
- Angela Lightbown, Katherine Crosby, Qingfu Zeng, Michael Lewis, Bradley L. Smith
352 KB PDF - Immunoafinity Profiling of Tyrosine Phosphorylation in Lung Cancer.
- Roberto D. Polakiewicz, Ailan Guo, Klarisa Rikova, Charles Farnsworth, Kim Lee, Al Moritz, John Rush, Michael Comb
246 KB PDF - Profiling Tyrosine Phosphorylation in Non Small Cell Lung Cancer.
- Klarisa Rikova, Ailan Guo, Qingfu Zeng, John Rush, Roberto Polakiewicz, Michael Comb
250 KB PDF
2004
- Analysis of Bcr/Abl signaling using large panels of phospho-sensitive antibodies.
- Randall K. Wetzel, Michael Peart, Bradley L. Smith
221 KB PDF
