Western Blotting and Immunoprecipitation
Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.
Our rigorous validation protocols help us provide specific and reliable antibodies, which helps you get reproducible and meaningful data every time.
Protocols: We offer detailed protocols so that you can start your experiments quickly and minimize time and reagent waste.
Technical Support: Our knowledgeable technical support scientists are a phone call or email away, so you have a partner at the bench.
Akt Western Blotting Example
Western blot of extracts from various cell lines, untreated or treated as indicated with LY294002 #9901 (a PI3 kinase inhibitor), Human Insulin-like Growth Factor I (hIGF-I) #8917 (which activates Akt via IGFIR binding), or Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (which induces the PI3K/Akt pathway through receptor binding), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (left) and Akt (pan) (C67E7) Rabbit mAb #4691 (right).
Immunoprecipitation (IP) is a technique used to enrich a specific protein from a heterogeneous cell or tissue extract using a target specific antibody. Co-immunoprecipitation (co-IP) is the pull down of intact protein complexes. IP and co-IP are valuable and widely used techniques to identify protein-protein interactions and novel members of protein complexes.
CST supplies high quality antibodies for IP and co-IP experiments that are validated using detailed protocols. The high degree of antibody specificity coupled with the availability of thoroughly validated companion products including IP beads and buffers ensure reliable IP and co-IP data to elucidate protein-protein interactions and protein complex composition. To save time and reagents, several antibodies are available conjugated to sepharose or magnetic beads for direct IP and co-IP experiments.
Custom conjugation of antibodies to sepharose and magnetic beads are available – please contact us for more information.