Sepharose and Magnetic Bead Conjugates

• Companion Products
• Protein A and G Beads
• Epitope Tag Antibodies
• Target-specific Antibodies

These antibodies are immobilized via covalent binding of primary amine groups to N-hydroxysuccinimide (NHS)-activated sepharose beads or by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead and are useful for immunoprecipitation. Please check back frequently for new conjugated antibodies. We will be adding more magnetic bead conjugates.

Benefits of Sepharose and Magnetic Bead Conjugates from Cell Signaling Technology

• Simplified immunoprecipitation protocol due to antibody immobilization.
• Minimized amounts of heavy and/or light chain release from the beads upon boiling in SDS sample buffer, which translates into a western blot with reduced contaminating heavy or light chain bands.
• Cell Signaling Technology's bead conjugates display maximum lot-to-lot consistency due to stringent quality control and titration.

Companion Products

New	No.	Name	Applications	Reactivity
	7017	6-Tube Magnetic Separation Rack
	3420	Mouse IgG (Sepharose Bead Conjugate)	IP
	5873	Mouse IgG (Magnetic Bead Conjugate)	IP
	3423	Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate)	IP
	3419	Streptavidin (Sepharose Bead Conjugate)	IP	All

Protein A and G Beads

New	No.	Name	Applications	Reactivity
	8687	Protein A Magnetic Beads	IP
	9863	Protein A Agarose Beads	IP
	8740	Protein G Magnetic Beads	IP
	9007	ChIP-Grade Protein G Agarose Beads	ChIP
	9006	ChIP-Grade Protein G Magnetic Beads	ChIP

Epitope Tag Antibodies

New	No.	Name	Applications	Reactivity
	3513	GST (26H1) Mouse mAb (Sepharose Bead Conjugate)	IP	All
	3956	HA-Tag (C29F4) Rabbit mAb (Sepharose Bead Conjugate)	IP
	4079	His-Tag (27E8) Mouse mAb (Sepharose Bead Conjugate)	IP	All
	3400	Myc-Tag (9B11) Mouse mAb (Sepharose Bead Conjugate)	IP	All
	5698	Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate)	IP

Target-specific Antibodies

New	No.	Name	Applications	Reactivity
New	No.	Name	Applications	Reactivity
	5939	AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk, (B, Dg)
	9563	Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Magnetic Bead Conjugate)	IP	H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg)
	4070	Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg)
	4343	Akt (pan) (C67E7) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk, Dm, (Pg)
	5697	Akt (pan) (40D4) Mouse mAb (Magnetic Bead Conjugate)	IP	H, M, R, Mk
	3653	Akt (pan) (40D4) Mouse mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	4090	Akt2 (D6G4) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	5611	ALK (C26G7) Rabbit mAb (Sepharose Bead Conjugate)	IP	H
	6707	AMPKα (23A3) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	5946	Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose Bead Conjugate)	IP	M
	6990	Anti-rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate)	IP
	3955	CREB (48H2) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	6625	Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (Sepharose Bead Conjugate)	IP	H, R, Mk
	5735	EGF Receptor (D38B1) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, Mk
	6937	EGF Receptor (D38B1) XP® Rabbit mAb (Magnetic Bead Conjugate)	IP	H, M, Mk
	8083	EGF Receptor (EGFR1) Mouse mAb (Sepharose Bead Conjugate)	IP	H
	9488	Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Magnetic Bead Conjugate)	IP	H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Sc, (Ce)
	3510	Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Hm, Mk, Mi, Dm, Z, B, Pg, Sc, (Dg)
	5736	p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Hm, Mk, Mi, Z, B, Dg, Pg, Ce
	5750	DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose Bead Conjugate)	IP
	5837	GβL (86B8) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	6938	GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate)	IP	H, M, R, Mk
	11995	GLI1 (L42B10) Mouse mAb (Sepharose Bead Conjugate)	IP	H
	4232	Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk, (X, Z)
	4088	Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk, Dg, (B, Pg)
	4078	IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Sepharose Bead Conjugate)	IP	H, M, R, Mk, B, Pg, GP
	4089	Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, (Mk)
	9219	Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate)	IP	H, M, R
	3551	Mer (D21F11) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H
	4091	Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R
	5838	Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, Mk
	6704	PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	4326	PTEN (D4.3) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	8095	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)	IP
	9419	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)	IP	All
	5501	Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate)	IP
	8726	Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate)	IP
	5382	Raptor (24C12) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	5379	Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M
	4306	Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Dm, Sc
	5167	Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M
	4074	Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk, (Hm, B, Pg, Hr)
	4368	Stat3 (79D7) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R, Mk
	9646	Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate)	IP	All
	8050	Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate)	IP
	3947	Survivin (71G4B7) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M, R
	8781	Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose Bead Conjugate)	IP
	5168	VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate)	IP	H, M

Applications Key: IP=Immunoprecipitation  ChIP=Chromatin Immunoprecipitation 
Reactivity Key: H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Mi=Mink  Dm=D.melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Sc=S.cerevisiae  GP=Guinea Pig  Hr=Horse  Ce=C.elegans  All=All Species Expected 
Species enclosed in parentheses are predicted to react based on 100% sequence homology.