Cell Signaling Technology

Organelle Markers

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Colocalization with one of the organelle-specific antibodies listed here can confirm the subcellular location of a protein of interest. While these organelle markers serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

To request a copy of our poster of Organelle Markers, please visit our request literature section; if you would like to download a copy of this poster, please visit our marketing materials section.

Expression of these proteins may vary in different cells and tissues.

Organelle Marker Antibody Sampler Kit

The Cellular Localization IF Antibody Sampler Kit #4753 provides an economical means for identification cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four Western blots with each antibody.

Atg12 Antibody (Human Specific) #2010 beta-Tubulin (9F3) Rabbit mAb #2128 Rab5 Antibody #2143 CENP-A Antibody #2186 Calnexin Antibody #2433 NUP98 (C39A3) Rabbit mAb #2598 Fibrillarin (C13C3) Rabbit mAb #2639 COX IV (3E11) Rabbit mAb #4850 Histone H3 Antibody #9715

The antibodies contained within the Cellular Localization IF Antibody Sampler Kit #4753 have been validated for IF-IC. This antibody sampler kit allows staining for the following organelles: Autophagosomes, Centromere, Cytoskeleton, Endoplasmic reticulum, Endosomes, Mitochondria, Nuclear Envelope, Nucleolus, and Nucleus.

Intercellular Junctions

No. Name Applications Reactivity
3195 E-Cadherin (24E10) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, (Dg)
3199 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, IF-P, F H, (M)
4065 E-Cadherin Antibody W, IP, IHC-P, IF-IC H, M, (B, Dg)
2189 P-Cadherin (C13F9) Rabbit mAb W, IF-IC H, (Mk)
2130 P-Cadherin Antibody W, IP, IF-IC, F H
2158 VE-Cadherin Antibody W, IF-IC H, B, Dm
3512 Connexin 43 Antibody W, IHC-P, IHC-F, IF-F, IF-IC H, M, R, Mk, Z, (Pg, Dg)
2847 ZO-2 Antibody W, IF-IC H, M, R, Mk, B, Dg

Cytoskeleton

No. Name Applications Reactivity
4545 Pan-Keratin (C11) Mouse mAb W, IHC-P, IF-IC, IF-P, F H, R, Mk
4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate) IF-IC, IF-P, F H, R, Mk
4528 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) IF-IC, IF-P, F H, R, Mk
4548 Keratin 18 (DC10) Mouse mAb W, IHC-P, IF-IC, F H
2125 α-Tubulin (11H10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, B, Dm, (Dg)
2144 α-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, B, Dm, (X)
2148 α/β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, B, Z
2128 β-Tubulin (9F3) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, B, Z, (C)
2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, R, Mk, Dm
2146 β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, B, Z, (X)

Cytoplasm

No. Name Applications Reactivity
9126 MEK1/2 (47E6) Rabbit mAb W, IF-IC, F H, M, R, Mk, (Dg)
4694 MEK1/2 (L38C12) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Mk

Ribosomes

No. Name Applications Reactivity
2403 Ribosomal Protein L7a (R225) Antibody W, IF-IC H, M, R, Mk
2579 Ribosomal Protein S3 Antibody W, IF-IC H, M, R, Mk
2317 S6 Ribosomal Protein (54D2) Mouse mAb W, IP, IHC-P, IF-IC, F H, M, R, Mk, Dm
2217 S6 Ribosomal Protein (5G10) Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk

Endoplasmic Reticulum

No. Name Applications Reactivity
2433 Calnexin Antibody W, IHC-P, IF-IC H
2446 PDI Antibody W, IHC-P, IF-IC H, M, R, Mk

Golgi

No. Name Applications Reactivity
2869 Syntaxin 6 (C34B2) Rabbit mAb W, IP, IF-IC H, M, R, Mk
2417 Syntaxin 6 Antibody W, IP, IF-IC H, M, R, Mk

Mitochondria

No. Name Applications Reactivity
4850 COX IV (3E11) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Pg, B, Z
4853 COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-F, IF-IC, F H, R, Mk, B
4844 COX IV Antibody W, IP, IHC-P, IF-IC, F H, M, R, Mk, B
4866 VDAC Antibody W, IHC-P H, M, R, B

Endosomes

No. Name Applications Reactivity
3238 Caveolin-1 Antibody W, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Pg, Hm, B, Z
2410 Clathrin Heavy Chain (P1663) Antibody W, IF-IC H, M, R, Mk, B
2411 EEA1 Antibody W, IP, IF-IC H, M, R, Mk
2145 NSF Antibody W, IP, IF-IC H, M, R, Mk, Hm
2143 Rab5 Antibody W, IF-IC H, M, R, Mk, Hm
2417 Syntaxin 6 Antibody W, IP, IF-IC H, M, R, Mk

Autophagosomes

No. Name Applications Reactivity
2010 Atg12 Antibody (Human Specific) W, IP, IF-IC H
2011 Atg12 Antibody (Mouse Specific) W, IP, IF-IC M
2775 LC3B Antibody W, IF-IC, F H, M, R, (Mk, C, B, X)

Nucleus

No. Name Applications Reactivity
2196 ESET (C1C12) Rabbit mAb W, IP, IF-IC H, Mk
2718 Histone H2A.Z Antibody W, IP, IF-IC H, M, R, Mk, Z, (C, B, X)
9715 Histone H3 Antibody W, IP, IHC-P, IF-IC H, M, R, Mk, Pg, B, Z, (Dm)
2184 LSD1 (C69G12) Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk
2139 LSD1 Antibody W, IP, IHC-P, IF-IC, F H, M, R, Mk

Nuclear Envelope

No. Name Applications Reactivity
2032 Lamin A/C Antibody W, IP, IHC-P, F H, M, R, (B)
2598 NUP98 (C39A3) Rabbit mAb W, IP, IF-IC H, M, R, Mk

Centrioles

No. Name Applications Reactivity
3258 PLK4 Antibody W, IF-IC H, M, R, (Mk)

Nucleolus

No. Name Applications Reactivity
2159 Fibrillarin (R122) Antibody (IF Specific) IF-IC Mk, (H, M, R)

Centromeres

No. Name Applications Reactivity
2187 Phospho-CENP-A (Ser7) Antibody W, IP, IF-IC H, (Mk)
2186 CENP-A Antibody W, IF-IC H

Mitosis

No. Name Applications Reactivity
3079 Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb W, IF-IC H
4718 Aurora A/AIK (1G4) Rabbit mAb W, IP, IF-IC H, Mk
2187 Phospho-CENP-A (Ser7) Antibody W, IP, IF-IC H, (Mk)
9701 Phospho-Histone H3 (Ser10) Antibody W, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Mk, Sc, C, Dm, Z, (X)
9708 Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate) IF-IC, F H, M, (X)
9706 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb W, IF-F, IF-IC, F H, M, R

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