Cell Signaling Technology is now offering a new highly sensitive and specific rabbit monoclonal antibody for the detection of HER3.
HER3/ErbB3 (D22C5) XP® Rabbit mAb #12708
Features & Benefits
- Highly sensitive antibody – allows detection of endogenous levels of HER3.
- No cross-reactivity with other family members – ensures specific detection of HER3 protein.
- Rabbit monoclonal antibody – eliminates background staining in mouse tissue typically seen with mouse monoclonal antibodies ensuring clean, consistent results.
- Validated in multiple applications – gives you flexibility in choosing the best application for your experiments.
HER3/ErbB3 (D22C5) XP® Rabbit mAb #12708 is a highly specific antibody approved for use in immunohistochemistry (IHC), immunofluorescence (IF), Flow Cytometry (F) and Western Blot (WB).
IHC analysis of paraffin-embedded MCF7 (Her3+; top) and MDA-MB-231 (HER3-; bottom) cell pellets.
IHC analysis of paraffin-embedded human breast (left), lung (center), and ovarian (right) carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb #12708.
Confocal immunofluorescent analysis of MCF7 cells (HER3+, left) and MDA-MB-231 cells (HER3-, right) using HER3/ErbB3 (D22C5) XP® Rabbit mAb #12708 (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of MCF7 cells using HER3/ErbB3 (D22C5) XP® Rabbit mAb #12708 (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor® 488 conjugate) #4412 was used as a secondary antibody.