Cell Signaling Technology (CST) scientists perform nearly one thousand western blots daily to validate our existing and new antibodies. Our protocol that has been optimized for over a decade is available online so you can replicate our procedure and get reproducible and reliable results.

Western Blotting Protocol

Our Western Blotting Protocol shows you the critical experimental steps in western blotting and explains how small changes to the protocol can affect the final outcome of your blot.

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Western Blotting Troubleshooting Guide

Our Western Blotting Troubleshooting Guide provides tips to help you diagnose problems and solutions to ensure you get the expected results in the shortest amount of time.

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