Comparison of SimpleChIP® Plus Chromatin IP Kits with a Kit from Another Supplier
Comparison of immunoprecipitated DNA relative to input chromatin (signal relative to input) obtained using the SimpleChIP® Plus Chromatin IP Kit #9005 to that obtained using the competitor kit demonstrates the increased immuno-enrichment and superior sensitivity of the SimpleChIP® Plus Kit.
Competitor Comparison of Tissue-based Chromatin IP Kits
Figure B. Competitor's Sonication-based Tissue ChIP Kit
SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic beads) #9005 (A) compared to a competitor’s sonication-based Tissue Chromatin IP kit (B). Mouse liver was cross-linked and disaggregated into a single-cell suspension using a tissue disaggregator. The chromatin was prepared and fragmented according to the manufacturer's recommendations and chromatin immunoprecipitations were performed using the indicated ChIP-validated antibodies. Purified DNA was analyzed by quantitative real-time PCR using SimpleChIP® Mouse GAPDH Intron 2 Primers #8986, SimpleChIP® Mouse AFM Intron 2 Primers #7269, SimpleChIP® Mouse HoxA1 Promoter Primers #7341, and SimpleChIP® Mouse HoxD10 Exon 1 Primers #7429. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to 1).