These positive and negative control cell extracts and proteins are made and used in-house for antibody validation and lot to lot testing. We recommend using these extracts as control lanes on your western blot, to ensure that western blotting procedures are appropriately executed. The use of proper controls also facilitates troubleshooting, as problems can then be attributed to either the western blotting protocol or the quality of the extracts used in the actual experiment.

For more information, please also see our controls table, which compiles cell lines and treatments that can be used as a positive control for our activation-state specific antibodies and serve as a reference for appropriately controlled experiments.

Control Cell Extracts

No. Name
No. Name
9273 Akt Control Cell Extracts
9158 AMPK Control Cell Extracts
9223 ATF-2 Control Cell Extracts
9263 c-Jun Control Cell Extracts
9663 Caspase-3 Control Cell Extracts
9193 CREB Control Cell Extracts
5634 EGF Receptor Control Cell Extracts
2043 Jurkat Apoptosis Cell Lysates (etoposide)
11972 LC3 Control Cell Extracts
9160 MEK1/2 HeLa Control Cell Extracts
9233 MKK3/MKK6 Control Cell Extracts
9243 NF-κB Control Cell Extracts
9213 p38 MAPK Control Cell Extracts
9194 p44/42 MAPK (Erk1/2) Control Cell Extracts
9203 p70 S6 Kinase Control Cell Extracts
7988 PathScan® Sandwich ELISA Control Phospho Cell Extract I
7989 PathScan® Sandwich ELISA Control Phospho Cell Extract II
9253 SAPK/JNK Control Cell Extracts
12052 Smad2/3 Control Cell Extracts
9173 Stat1 Control Cell Extracts
9133 Stat3 Control Cell Extracts
9353 Stat5 Control Cell Extracts

Control Proteins

No. Name
No. Name
9293 Bad Control Proteins
9113 cdc2 (Tyr15) Control Proteins
9183 Elk-1 Control Proteins
9103 p44/42 MAPK (Erk1/2) Control Proteins
9303 Rb Control Proteins
2904 VEGF Receptor 2 Control Proteins