Phospholipid Binding: CALM Domain

The CALM/ANTH domain of AP180 bound to PtdIns(4,5)P2

Domain Binding and Function

The CALM (Clathrin Assembly Lymphoid Myeloid) domain, also known as ANTH, has a structure similar to the ENTH domain apart from the addition of one or more α-helices. Although both the CALM and ENTH domains bind PtdIns(4,5)P2 with high specificity, they differ in the manner in which they bind. ENTH domains bind PtdIns(4,5)P2 within a pocket, while ANTH domains lack a well defined binding pocket and instead use surface exposed basic residues to coordinate binding. CALM domain containing proteins include AP180 and CALM, which are implicated in the formation of clathrin-coated pits.

Structure Reference

  1. Ford, M.G. et al. (2001) Science 291(5506), 1051–1055

Examples of Domain Proteins

Phospholipid Binding: CALM Domain

Binding Examples

CALM/ANTH Domain Proteins Binding Partners
AP180 PtdIns(4,5)P2
CALM (AP180-2) PtdIns(4,5)P2