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Our scientists are at the bench daily to produce and validate our antibodies, so they have hands-on experience and knowledge of each antibody’s performance.

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Starting with the PathScan® Sandwich ELISA kits listed below, the detection antibody and HRP-conjugated secondary antibody in each of these kits will be packaged in a lyophilized format rather than a traditional liquid format. This format enhancement was driven by a request to increase the shelf life of these kits from 6 months to 1 year from the reference date listed on each kit. An extended shelf life provides superior signal retention over time and maximum flexibility in experimental design. The longer shelf life also streamlines production and helps ensure that your product will be available when you need it.

Matched High Performance Between Kits Containing Lyophilized or Traditional Antibodies

ELISA Figure 1 Positive ELISA Figure 2 Negative

Figure 1: PathScan® Phospho-NF-κB p65 (Ser536) Sandwich ELISA Kit #7173 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from Jurkat cells treated with calyculin A was used as a positive control (A), and signal was measured across a range of sample titration points. A lysate prepared from untreated Jurkat cells was tested as a negative control (B).

ELISA Figure 2

Figure 2: PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit #7976 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from Jurkat cells treated with calyculin A was used as a positive control, and signal was measured across a range of sample titration points.

ELISA Figure 3

Figure 3: PathScan® Phospho-TrkA (Tyr490) Sandwich ELISA Kit #7210 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from 3T3/TrkA cells treated with NGF was used as a positive control, and signal was measured across a range of sample titration points.

ELISA Figure 4

Figure 4: PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Kit #7167 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from untreated 293 cells was used as a positive control, and signal was measured across a range of sample titration points.

Effected PathScan® Sandwich ELISA Kits

No. PathScan® Sandwich ELISA Kit
No. PathScan® Sandwich ELISA Kit
12070C PathScan® Phospho-c-Abl (Tyr412) Sandwich ELISA Kit
7135C PathScan® Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit
7160C PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit
7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit
7170C PathScan® Total Akt1 Sandwich ELISA Kit
7930C PathScan® Total Akt2 Sandwich ELISA Kit (Mouse Preferred)
7959C PathScan® Phospho-AMPKα (Thr172) Sandwich ELISA Kit
7190C PathScan® Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit
7863C PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit
7240C PathScan® Phospho-EGF Receptor (Tyr1068) Sandwich ELISA Kit
7250C PathScan® Total EGF Receptor Sandwich ELISA Kit
7952C PathScan® Total eIF2α Sandwich ELISA Kit
7177C PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit
7761C PathScan® Phospho-FLT3 (panTyr) Sandwich ELISA Kit
7123C PathScan® Mono-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit
7125C PathScan® Tri-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit
7866C PathScan® Tri-Methyl-Histone H3 (Lys27) Sandwich ELISA Kit
7124C PathScan® Di-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit
7253C PathScan® Total Histone H3 Sandwich ELISA Kit
7152C PathScan® Phospho-HSP27 (Ser82) Sandwich ELISA Kit
7078C PathScan® Total IKKα Sandwich ELISA Kit
7258C PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Kit
7326C PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Kit
7231C PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Kit
7298C PathScan® Phospho-c-Kit (Tyr719) Sandwich ELISA Kit
7175C PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit
7029C PathScan® Phospho-MEK1 (Ser217/221) Chemiluminescent Sandwich ELISA Kit
7896C PathScan® Phospho-Met (Tyr1349) Sandwich ELISA Kit
7976C PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit
7974C PathScan® Total mTOR Sandwich ELISA Kit
7173C PathScan® Phospho-NF-κB p65 (Ser536) Sandwich ELISA Kit
7167C PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Kit
7296C PathScan® Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit
7093C PathScan® Phospho-ROS1 (panTyr) Sandwich ELISA Kit
7091C PathScan® Total ROS1 Sandwich ELISA Kit
7348C PathScan® Phospho-Smad2 (Ser465/467) Sandwich ELISA Kit
7244C PathScan® Total Smad2 Sandwich ELISA Kit
7149C PathScan® Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit
7300C PathScan® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit
7305C PathScan® Total Stat3 Sandwich ELISA Kit
7210C PathScan® Phospho-TrkA (Tyr490) Sandwich ELISA Kit
7335C PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit
7172C PathScan® Total Zap-70 Sandwich ELISA Kit