Loading controls are used to ensure equal loading of a gel and are important when western blot results are being compared qualitatively or quantitatively. Loading controls often used are proteins which express well across many cell lines and tissues, these include targets such as: β-Actin, COX IV, GAPDH, Histone H3, and α- and β-Tubulin.

Loading Control Antibody Sampler Kits

No. Name
No. Name
5142 Loading Control Antibody Sampler Kit
9774 Loading Control Antibody Sampler Kit (Mouse)
4670 Loading Control Antibody Sampler Kit (HRP Conjugate)

Loading Control Antibodies

No. Name Applications Reactivity
No. Name Applications Reactivity
12620 β-Actin (D6A8) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, (Hm, C)
12748 Pan-Actin (D18C11) Rabbit mAb (HRP Conjugate) W H, M, R, Mk
4970 β-Actin (13E5) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
5125 β-Actin (13E5) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, B, Pg, (C, Hr)
12262 β-Actin (8H10D10) Mouse mAb (HRP Conjugate) W H, M, R, Hm, Mk, Dg
3700 β-Actin (8H10D10) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Hm, Mk, Dg
4967 β-Actin Antibody W H, M, R, Hm, Mk, Mi, Dm, Z, B, (C, X, Dg, Pg, Hr)
5247 COX IV (3E11) Rabbit mAb (HRP Conjugate) W H, Mk
4850 COX IV (3E11) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg
4844 COX IV Antibody W, IP, IHC-P, F H, M, R, Mk, B
8884 GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate) W H, M, R, Mk
5174 GAPDH (D16H11) XP® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk, (Pg)
2118 GAPDH (14C10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, B, (Pg)
5014 GAPDH (14C10) Rabbit mAb (Biotinylated) W, IF-IC, F H, M, R, Mk
3683 GAPDH (14C10) Rabbit mAb (HRP Conjugate) W H, M, R, Mk
4499 Histone H3 (D1H2) XP® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk, (Hm, C, Dm, X, Z, B)
5192 Histone H3 (3H1) Rabbit mAb (HRP Conjugate) W H, M, R, Hm, Mk, Z, B, Pg
9717 Histone H3 (3H1) Rabbit mAb W H, M, R, Hm, Mk, Dm, Z, B, Dg, Pg
3638 Histone H3 (96C10) Mouse mAb W H, M, R, Mk, (Dm, X, Hr)
9715 Histone H3 Antibody W H, M, R, Mk, Z, B, Pg, (Dm)
5346 β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, Z, B, (C)
2128 β-Tubulin (9F3) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (C)
2146 β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (X)
2148 α/β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B
9099 α-Tubulin (11H10) Rabbit mAb (HRP Conjugate) W H, M, R, Mk, Dm, B, (Dg)
2125 α-Tubulin (11H10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (Dg, Pg)
3873 α-Tubulin (DM1A) Mouse mAb W, IHC-P, IF-IC H, M, R, Mk
12351 α-Tubulin (DM1A) Mouse mAb (HRP Conjugate) W H, M, R, Mk
2144 α-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (X)

Applications Key: W=Western blot  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow cytometry 
Reactivity Key: H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Mi=Mink  Dm=D.melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Hr=Horse 
Species enclosed in parentheses are predicted to react based on 100% sequence homology.