KinomeView<sup>®</sup> Profiling

KinomeView® Profiling is a western blot based prescreening tool that uses the same motif and PTM antibodies available for PTMScan® analysis.

KinomeView Profiling enables you to:

  • Observe global signaling changes across multiple samples.
  • Select the best antibodies for use in subsequent PTMScan Discovery experiments.
  • Optimize experimental conditions (treatment dose, time-course, cell line, or tissue, etc.).

The KinomeView® Profiling Kit #9812 provides motif antibodies for a kinome-wide view of cellular phosphorylation. The KinomeView® Profiling Service includes motif antibodies for studying changes in phosphorylation and additional PTMs. The service is done by CST scientists and includes data analysis and expert project consultation. Please inquire for KinomeView® Profiling Services.

KinomeView® Profiling Kit and Services

KinomeView Kit/Services for Phosphorylation Proteomics

Antibody Description Motif Kit #9812 Service
Antibody Description Motif Kit #9812 Service
14-3-3 Binding Motif (R/K)XX(s/t)XP
Akt Substrate RXX(s/t)
Akt Substrate RXRXX(s/t)
AMPK Substrate LXRXX(s/t)
ATM/ATR Substrate (s/t)Q
ATM/ATR Substrate (s/t)QG
CDK Substrate (K/R)(s/t)PX(K/R)
CK2 Substrate (s/t)(D/E)X(D/E)
MAPK/CDK Substrate PX(s/t)P, (s/t)PX(K/R)
PDK1 Docking Motif (F/K)XX(F/Y)(s/t)F/Y)
PKA Substrate (K/R)(K/R)X(s/t)
PKC Substrate (K/R)X(s/t)X(K/R)
PKD Substrate LXRXX(s/t)
PLK Binding Motif S(s/t)P
tP Motif (s/t)P, (s/t)PP
tPE Motif (s/t)PE
tXR/tPR Motif (s/t)XR, (s/t)PR
Phospho-Tyrosine (pY-1000) y

Western-blot prescreen for other post-translational modifications

Antibody Description Modification Motif Service
Antibody Description Modification Motif Service
Mono-Methyl Arginine Methylation Me-R
Asymmetric Di-Methyl Arginine Methylation adme-RG
Symmetric Di-Methyl Arginine Methylation sdme-RG
Acetyl-Lysine Acylation Ac-K
Glutaryl-Lysine Acylation Glut-K
Malonyl-Lysine Acylation Mal-K
Propionyl-Lysine Acylation Prop-K
Caspase Cleavage Substrate Proteolytic cleavage [DE(T/S/A)D]

Illustration of Motif Antibody Coverage

Kinome Target #1
Kinome Target #2a
Kinome Target #2b
Kinome Target #4a
Kinome Target #4b
Kinome Target #5a
Kinome Target #5b
Kinome Target #6a
Kinome Target #6b
Kinome Target #7
Kinome Target #8
Kinome Target #9
Kinome Target #10
Kinome Target #11
Kinome Target #12
KinomeView Profiling pY pS/pT Akt Motif K/RK/RXpS/pT pS/T Akt Motif RXRXXpS/pT pT Motif pTXR pS PKC Motif K/RXpSXK/R pT MAPK Motif PXpTP pS MAPK Motif PXpSP pS/pT ATM Motif pS/pTQG pS/pT ATM Motif pS/pTQ pT Motif pTP pS/T PKD Motif LXRXXpS/pT pT CK Motif pS/pTD/EXD/E pS/pT PKA Motif K/RK/RXpS/pT pS/pT PDK1 Docking Motif F/YpS/pTF/Y pS CDK Motif K/RpSPXK/R