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PTMScan® Motif Antibody Kits
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Cell Signaling Technology developed Motif Antibodies to enable focused analysis of regulatory post-translational modifications (PTMs). We use these in the form of custom PTMScan License/IAP Bead Products in our PTMScan Discovery Services, and we provide these products with a Per-Use License to enable our customers to perform PTMScan proteomics in-house.

Whether your research objective is to understand specific serine/threonine kinase families or kinome branches, global tyrosine phosphorylation, protein acetylation, or ubiquitin-mediated signaling, our PTMScan Discovery Services and PTMScan License/IAP Beads products provide powerful tools for in-depth proteomic analysis of regulatory PTMs.

PTMScan – PhosphoScan®, UbiScan®, AcetylScan® – is the most effective strategy for PTM proteomics, and our PTMScan License/IAP Bead products (see below) are custom, optimized reagents for immunoaffinity purification (IAP) of serine/threonine and tyrosine phosphopeptides, acetylated peptides and ubiquitin-tagged peptides. PTMScan is a method that combines Motif Antibody-based IAP and tandem mass spectrometry to generate focused and quantitative results about proteins on which PTMs perform key regulatory functions. It is the PTM-specificity of our Motif Antibodies that makes PTMScan Discovery Services and Per-Use Licensing of PTMScan License/IAP Bead products the standards for proteomic analysis of regulatory signaling.

For each of the motif antibodies listed in the table below, a RAW LC-MS/MS data file is provided. The LC-MS/MS data file was generated using a Thermo Orbitrap (Velos or Elite) mass spectrometer. In addition, a result table (in excel format) is accompanied with each RAW data file generated from the corresponding motif antibody enrichment of mouse liver peptides. The peptide/protein assignments in the accompanying excel table were produced using SEQUEST. The sample description for each data set is provided in the header of the results table.

KinomeView® and Serine/Threonine PhosphoScan® Products

Description Antibody Kit Consenus Site LCMS Data & Output Results
Description Antibody Kit Consenus Site LCMS Data & Output Results
Phospho-Akt Substrate 9614 5561 RXX(S*/T*) Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-Akt Substrate 10001 5563 RXRXX(S*/T*) Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-(Ser/Thr) AMPK Substrate 5759 5564 (L/M)XRXX(S*/T*), RXX(S*/T*) Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-(Ser) ATM/ATR Substrate 9607 12267 S*Q Mouse Liver  |  XLS  |  RAW
Phospho-(Ser/Thr) ATM/ATR Substrate 6966 12267 (S*/T*)QG, (S*/T*)Q Mouse Liver  |  XLS  |  RAW
Phospho-(Ser) CDKs Substrate 9477   (K/H)S*P  
Phospho-(Ser/Thr) CK II Substrate 8738 12170 (S*/T*)DXE Mouse Liver  |  PDF
Phospho-MAPK/CDK Substrates 2325 4652 PXS*P, S*PX(K/R) Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-(Ser/Thr) PDK1 Docking Motif 9634   (F/K)XX(F/Y)(S*/T*)(F/Y) Mouse Liver  |  XLS  |  RAW
Phospho-PKA Substrate 9624 5565 (K/R)(K/R)X(S*/T*) Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-(Ser) PKC Substrate 6967   (K/R)XS*X(K/R) Mouse Liver  |  XLS  |  RAW
Phospho-(Thr) PLK Binding Motif 5243 5566 ST*P Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-Thr-Pro Motif 3003   T*P, T*PP Mouse Liver  |  XLS  |  RAW
Phospho-Thr-Pro-Glu Motif 3004   T*PE, T*P Mouse Liver  |  XLS  |  RAW  |  PDF
Phospho-Thr-X-Arg Motif 2351   T*X(K/R) Mouse Liver  |  XLS  |  RAW  |  PDF

Tyrosine PhosphoScan® Products

Description Antibody Kit Consenus Site LCMS Data & Output Results
Phospho-Tyrosine (P-Tyr-100) 9411 5636 Y* Mouse Brain  |  XLS  |  RAW  |  PDF
Phospho-Tyrosine (P-Tyr-1000) 8954 8803 Y* Mouse Brain  |  XLS  |  RAW

AcetylScan® Products

Description Antibody Kit Consenus Site LCMS Data & Output Results
Acetylated-Lysine 9814 13416 Ac-K Mouse Liver  |  XLS  |  RAW

Cleaved Caspase Substrate PTMScan® Products

Description Antibody Kit Consenus Site LCMS Data & Output Results
Cleaved Caspase Substrate 8698 12810 DE(T/S/A)D  

MethylScan® Products

Description Antibody Kit Consenus Site LCMS Data & Output Results
Mono-Methylated-Arginine 8015, 8711 12235 Me-R and Me-RGG Mouse Embryo  |  XLS  |  RAW  |  PDF
Di-Methyl Arginine 13522 13474 [R]  
Di-Methyl Arginine 13222 13563 [RG]  

UbiScan® Products

Description Antibody Kit Consenus Site LCMS Data & Output Results
Ubiquitin Branch 3925 5562 K-ε-GG Mouse Liver  |  XLS  |  RAW  |  PDF