PTMScan® Proteomics Services from Cell Signaling Technology (CST) employ proprietary methodologies for antibody-based peptide enrichment combined with tandem mass spectrometry for quantitative profiling of post-translational modifications (PTMs), including phosphorylation, ubiquitination, methylation and acetylation.

PTMScan® Discovery Services

PTMScan® Discovery Services are intended as a discovery-mode proteomics technology for identifying and quantifying post-translational modifications in biological model systems.
Overview of PTMScan Discovery >>

Why consider antibody enrichment for studying phosphorylation?

  • Focused discovery of phosphosites based on amino acid (S/T/Y) or motif of interest
  • Increased coverage of lower occurring phosphorylation events compared to IMAC
  • View data showing complementarity between antibody enrichment and IMAC

PTMScan® Direct Services

PTMScan® Direct Services are intended as a targeted-mode proteomics screening technology with defined assay endpoints, allowing focused studies on critical signaling nodes.
Overview of PTMScan Direct >>

Proteomics of post-translational modification example.

 

Sample Preparation Protocols

Before sample preparation for PTMScan® Discovery, KinomeView®, or PTMScan® Direct Services, please download and review the PTMScan or KinomeView Sample Preparation Protocols.

Services Timeline

Estimated Completion Times for PTMScan® Projects

Number of Samples 1 Motif Ab (Weeks) 2 Motif Abs (Weeks) 3 Motif Abs (Weeks)
1–4 4–6 5–7 6–8
5–8 5–7 6–8 7–9
8–16 6–8 7–9 8–10
16–24 7–9 8–10 9–11
24–42 8–10 9–11 11–13
greater than 42* 9–11 11–13 13–15

Estimated Completion Times for KinomeView® Projects

Number of Lanes ~20 Motif Ab + 3 Control Abs (Weeks)
2–6 2–3
7–14 3–4
15–28 3–5
29–42 4–6
greater than 42* 5–8

*More accurate timelines for service projects with greater than 42 samples will be discussed during the consultation. NOTE: Estimated timelines are based on when the project is initiated by Cell Signaling Technology, not when the material is received from the client.