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Technical Support

Our scientists are at the bench daily to produce and validate our antibodies, so they have hands-on experience and knowledge of each antibody’s performance.

Contact a Scientist

To ensure a timely solution, please have the information below ready when you contact us.

Have you consulted our Immunohistochemistry Troubleshooting Guide before using this technical report?

Tissue

  1. Species.
  2. Organ.
  3. Fixation (drop fixed, perfused, or unfixed).
    1. Fixative used
    2. Time
    3. Temperature
  4. Sections (paraffin-embedded, fixed cryosections, fresh frozen, floating).

Antigen Retrieval

  1. Buffer.
  2. Describe procedure (time, temperatures, apparatus, etc.).

Hydrogen Peroxide Quench Performed?

  1. Buffer.
  2. Time.

Block

  1. Buffer.
  2. Time.
  3. Temperature.

Primary Antibody

  1. Catalog number.
  2. Lot number and reference date.
  3. Dilution.
  4. Diluent.
  5. Length of incubation.
  6. Temperature of incubation.

Wash Buffer

Secondary Reagents

  1. Manufacturer.
  2. Dilution.
  3. Time.
  4. Temperature.

Substrate

  1. Name.
  2. Length of incubation.

Results

  1. Localization/background.

Controls

  1. Negative used and result.
  2. Positive used and result.