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Scientists from Cell Signaling Technology regularly present posters at scientific conferences. Our studies involve the development of new technologies for signaling pathway analysis and mechanistic cell biology research.

Portable Document Format (PDF) files of our investigative research are linked below. Some of the PDFs are large files. The sizes for each file are listed.

Mapping deregulated pathways downstream of RTKs in cell line models.
Klarisa Rikova, Benjamin Hall, Anthony Possemato, Keri Mroszczyk, Kimberly A. Lee, Jian Min Ren, Ailan Guo, Daniel Mulhern, Yi Wang, Sean A. Beausoleil, Michael J. Comb
2.8 MB PDF
Understanding of Differing Sensitivity in EML4-ALK NSCLC Patients to Crizotinib and Geldanamycin.
Klarisa Rikova, Benjamin Hall, Anthony Possemato, Keri Mroszczyk, Kimberly A. Lee, Joan MacNeill, Jian Min Ren, Ailan Guo, Daniel Mulhern, Yi Wang, Sean A. Beausoleil, Michael J. Comb
3.9 MB PDF
Proteomic Based Analysis of Ovarian Cancer Pathways.
Klarisa Rikova, Benjamin Hall, Tyler Levy, Caroline Keroack, Anthony Possemato, Kimberly A. Lee, Joan MacNeill, Jian Min Ren, Ailan Guo, Daniel Mulhern, Yi Wang, Sean A. Beausoleil, Scott Lonning, Michael J. Comb
1.2 MB PDF
Probing EGFR, HER2, and c-Met Protein-Protein Interactions Using an Antibody Array.
Jacob Knowles, Stephen Capodilupo, Richard Noring, Roberto Campos-González, Julio Herrera and Zev Gechtman
2.1 MB PDF
Motif Antibody Enrichment Enables the Identification of a Large Discrete and Complementary Set of Phosphorylation Sites.
Anthony Possemato; Mike Aguiar, Kim Lee, Steven P. Gygi, Sean A. Beausoleil
3.5 MB PDF
Quantitative Profiling of the Acetylome in Mouse Tissue using Lysine Acetylation Reagents in Immunoaffinity LC-MS/MS.
Hongbo Gu, Matthew P. Stokes, Ailan Guo, Kimberly Lee, Jian Min Ren, Xiaoying Jia, Vipin Suri, Jeffrey Silva
3.4 MB PDF
Network and Pathway Analysis of Post-translationally Modified Peptides Identified by Immunoaffinity-based Proteomics.
Jeffrey C. Silva, Stuart Tugendreich, Hongbo Gu, Charles L. Farnsworth, Kimberly A. Lee, Xiaoying Jia, Jian Min Ren, Matthew P. Stokes
6.9 MB PDF
Development and application of highly sensitive SIM and SRM-based phosphopeptide immunoassays using the Q Exactive™ mass spectrometer.
Kimberly A. Lee, Joan MacNeill, Jing Zhou, Jian Yu, Matthew P. Stokes, Jeffrey C. Silva, Ailan Guo, Michael J. Comb
4.4 MB PDF
Quantitative profiling of signaling pathways using immunoaffinity purification and LC-MS/MS.
Matthew P. Stokes, Jian Min Ren, Kimberly A. Lee, Xiaoying Jia, Jeffrey C. Silva
20.5 MB PDF
Quantitative Profiling of the Acetylome in Mouse Tissue using Lysine Acetylation Reagents in Immunoaffinity LC-MS/MS.
Bryon F. Ricketts, Hongbo Gu, Matthew P. Stokes, Ailan Guo, Kimberly Lee, Jian Min Ren, Xiaoying Jia, Vipin Suri, Jeffrey Silva
4.2 MB PDF
Monitoring the evolution of antigen-specific antibodies in circulation and their corresponding B cell clones in longitudinal immunization case studies through the use of NG-XMT™.
Jaime R. Darce, Stephen R. Lutz, Shuji Sato, Jason G. Beaudet, Michael Palazzola, Ivan Gregoretti, Sasha Tkachev, Sean A. Beausoleil, Lana Popova, Ravi K. Ramenani, Elizabeth Moreno, Xiaowu Zhang, James S. Wieler, Sandra M. Schieferl, Roberto D. Polakiewicz, and Wan Cheung Cheung
2.9 MB PDF
Highly Parallel Planar Surface Antibody Array-based Assay to Monitor Protein-protein Interactions.
Jacob Knowles, Stephen Capodilupo, Richard Noring, Roberto Campos-González, Julio E. Herrera, and Zev Gechtman
1.2 MB PDF
Proteomic Characterization of the Evolution of the Circulating Antibody Response to Hepatitis B Virus Vaccination.
Shuji Sato,, Sean A. Beausoleil,, Lana Popova, Jason G. Beaudet, Ravi K. Ramenani, Xiaowu Zhang, James S. Wieler, Albrecht Moritz, Sandra M. Schieferl, Wan Cheung Cheung, and Roberto D. Polakiewicz
3.4 MB PDF

2012 Posters

Comprehensive Quantitative Profiling of the Mouse Kinome using Data-independent LC-MS-MS/MSALL with SWATH™ Acquisition.
Jeffrey C. Silva, Matthew P. Stokes, Charles L. Farnsworth, Xiaoying Jia, Christie Hunter
909 KB PDF
Quantitative Profiling of Signaling Pathways Using PTMScan® Direct.
Matthew P. Stokes, Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Roberto Polakiewicz, Michael J Comb
2.2 MB PDF
The Large-scale Analysis of Protein Expression in Human Ovarian Tumor Samples by LC-MS/MS/MS.
Anthony Possemato, Sean Beausoleil, Klarisa Rikova, Hong Ren, Kimberly Lee, Michael Comb
1.7 MB PDF
Multiplexing Kinase Substrate Motif Antibodies for Expanded Coverage of the Serine/Threonine Kinome.
Charles L. Farnsworth, Jeffrey C. Silva, Matthew P. Stokes, Hongbo Gu, Ailan Guo, Kimberly A. Lee, Jian-Min Ren, Xiaoying Jia, Roberto D. Polakiewicz, Michael J. Comb
717 KB PDF
Immunoaffinity Enrichment and Quantitative Profiling of Protein Phosphorylation, Methylation, and Acetylation in Receptor Tyrosine Kinase Pathway-addicted Cancer Cell Lines.
Klarisa Rikova, Ailan Guo, Kimberly A. Lee, Anthony Possemato, Yi Wang, Sean A. Beausoleil, Daniel E. Mulhern, Jian Min Ren, Michael J. Comb
2.5 MB PDF
A Proteomics Approach for the Identification and Cloning of Monoclonal Antibodies from Serum.
Wan Cheung (Gabriel) Cheung*, Sean A. Beausoleil*, Xiaowu Zhang, Shuji Sato, Sandra M. Schieferl, James S. Wieler, Jason G. Beaudet, Ravi K. Ramenani, Lana Popova, Michael J. Comb, John Rush, and Roberto D. Polakiewicz.
*These authors contributed equally to this work
1.4 MB PDF
Over-expression of EphB1 leads to Stat3 phosphorylation.
Danielle Swain, Richard Noring, Jake Knowles, Roberto Campos-González, Zev Gechtman
1.1 MB PDF
Rapid quantification of autophagy in cell-based assays.
Christopher A. Manning, Michael R. Nelson, Paul Wylie, Diana Caracino, Randall K. Wetzel
1.9 MB PDF

2011 Posters

The use of a four-color multiplex antibody-based assay with laser-scanning high content screening to examine kinase inhibitor effects on cellular signaling.
Jessica A. Cherry, Paul Wylie, Christopher A. Manning, Diana Caracino, Randall K. Wetzel
596 KB PDF
PTMScan® Direct: Quantitative Profiling of Critical Nodes From Cellular Signaling Pathways.
Matthew P. Stokes, Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Albrecht Moritz, Ailan Guo, John Rush, Roberto Polakiewicz, Michael J. Comb
8.1 MB PDF

2008 Posters

Mutation-Specific Antibodies for the Detection of EGFR Mutations in Non-Small-Cell Lung Cancer.
Jian Yu, Susan Kane, Daiqiang Li, Herbert Haack, Bradley Smith, Ting-Lei Gu, Massimo Loda, Xinmin Zhou, Michael J. Comb
1.6 MB PDF
Analysis of kinase inhibitors using a large array of signaling antibodies and high content screening.
Jessica A. Cherry, Corinne Michaud, Paul Wylie, Randall K. Wetzel
852 KB PDF

2007 Posters

Evaluation of PTEN: Immunohistochemical Analysis of Tissues and Cells.
Angela Lightbown, Qingfu Zeng, Michael Lewis, Katherine Crosby, Bradley L. Smith
360 KB PDF
Detection of Genetic Abnormalities in Cancer Cells using Bio-Plex® Immuno-Assays.
Andrew Romano, Kathleen Rogers, Susan Kane, Jeff Lee, Whei Feng, Bradley L. Smith, Reggie Prioli
246 KB PDF
Phosphorylation Signatures Identify Tyrosine Kinases Activated in Lung Cancer.
Klarisa Rikova, Ailan Guo, Qingfu Zeng, Anthony Possemato, Herbert Haack, Julie Nardone, Kimberly Lee, Yu Li, Laura Sullivan, Joan MacNeill, Corey Bakalarski, Judit Villen, John Kornhauser, John Rush, Steven Gygi, Roberto Polakiewicz, Michael Comb
822 KB PDF
Rapid and Quantitative Cell-Based Immunoaffinity Kinase Assay in a 96-well Format.
Jin Yuan, Craig Thompson
569 KB PDF
The Detection of Phosphoproteins by Bio-Plex® Assays in Cell, Xenograft and Human Tumor Lysate.
Jeremy Fisher, Kathleen Rogers, Katherine Crosby, Andrew Romano, Bradley L. Smith, Reginaldo Prioli
512 KB PDF
PhosphoSite®: A Knowledge Base of Post-Translational Modifications and Their Role in Cell Signaling.
David Merberg, Roland Carel, Jon Kornhauser, Juan Munoz, Beth Murray, Julie Nardone, Ousama Shamma, Elzbieta Skrzypek, Bin Zhang, Peter Hornbeck
1.1 MB PDF
Phospho-Specific Rabbit Monoclonal Antibodies Validated for Immunohistochemistry.
Cynthia Shapiro, Angela Lightbown, Kathleen Rogers, Jessica Simendinger, Michael Lewis, Katherine Crosby
2.9 MB PDF