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Our scientists are at the bench daily to produce and validate our antibodies, so they have hands-on experience and knowledge of each antibody’s performance.

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Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370, and Jak2 (D2E12) XP® Rabbit mAb #3230 exemplify the consistent performance of exhibited by every XP® monoclonal antibodies from lot to lot. Each new lot is compared to the previous lot for all recommended applications before it becomes available to our customers, which ensures the greatest possible consistency in research results.

XP® Reproducibility Comparison featuring p44/42 MAPK (Erk1/2) #4370

IHC analysis of adjacent sections of paraffin-embedded human colon carcinoma Figure 2. IHC analysis of adjacent sections of paraffin-embedded human colon carcinoma using #4370 lot 7 at a 1:400 dilution (upper left), #4370 lot 9 at a 1:200 dilution (upper right), #4370 lot 9 at a 1:400 dilution (lower left), and #4370 lot 9 at a 1:800 dilution (lower right). The recommended dilution for lot 9 remained 1:400.

XP® Reproducibility Comparison featuring Jak2 #3230

Western blot analysis of HeLa cells, untreated or treated with IFN-a. Figure 3. Western blot analysis of K562 cells comparing lots 1, 3, and 5 of #3230, showing that signal remains consistent from lot to lot.