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Technical Support

Our scientists are at the bench daily to produce and validate our antibodies, so they have hands-on experience and knowledge of each antibody’s performance.

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Starting with the PathScan® Sandwich ELISA kits listed below, the detection antibody and HRP-conjugated secondary antibody in each of these kits will be packaged in a lyophilized format rather than a traditional liquid format. This format enhancement was driven by a request to increase the shelf life of these kits from 6 months to 1 year from the reference date listed on each kit. An extended shelf life provides superior signal retention over time and maximum flexibility in experimental design. The longer shelf life also streamlines production and helps ensure that your product will be available when you need it.

Matched High Performance Between Kits Containing Lyophilized or Traditional Antibodies

ELISA Figure 1 Positive ELISA Figure 2 Negative

Figure 1: PathScan® Phospho-NF-κB p65 (Ser536) Sandwich ELISA Kit #7173 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from Jurkat cells treated with calyculin A was used as a positive control (A), and signal was measured across a range of sample titration points. A lysate prepared from untreated Jurkat cells was tested as a negative control (B).

ELISA Figure 2

Figure 2: PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit #7976 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from Jurkat cells treated with calyculin A was used as a positive control, and signal was measured across a range of sample titration points.

ELISA Figure 3

Figure 3: PathScan® Phospho-TrkA (Tyr490) Sandwich ELISA Kit #7210 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from 3T3/TrkA cells treated with NGF was used as a positive control, and signal was measured across a range of sample titration points.

ELISA Figure 4

Figure 4: PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Kit #7167 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from untreated 293 cells was used as a positive control, and signal was measured across a range of sample titration points.

Effected PathScan® Sandwich ELISA Kits

No. PathScan® Sandwich ELISA Kit
No. PathScan® Sandwich ELISA Kit
7216C PathScan® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit
7179C PathScan® Total 4E-BP1 Sandwich ELISA Kit
7903C PathScan® Phospho-c-Abl (panTyr) Sandwich ELISA Kit
12070C PathScan® Phospho-c-Abl (Tyr412) Sandwich ELISA Kit
12116C PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit
7986C PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit
7996C PathScan® Total Acetyl-CoA Carboxylase Sandwich ELISA Kit
7880C PathScan® Total β-Actin Sandwich ELISA Kit
7252C PathScan® Phospho-Akt (Thr308) Sandwich ELISA Kit
7135C PathScan® Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit
7160C PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit
7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit
7170C PathScan® Total Akt1 Sandwich ELISA Kit
7048C PathScan® Phospho-Akt2 (Ser474) Sandwich ELISA Kit
7932C PathScan® Phospho-Akt2 (Ser474) Sandwich ELISA Kit (Mouse Preferred)
7046C PathScan® Total Akt2 Sandwich ELISA Kit
7930C PathScan® Total Akt2 Sandwich ELISA Kit (Mouse Preferred)
7942C PathScan® Phospho-Akt3 (Ser472) Sandwich ELISA Kit (Mouse Preferred)
7934C PathScan® Total Akt3 Sandwich ELISA Kit (Mouse Preferred)
7159C PathScan® Phospho-ALK (Tyr1586) Sandwich ELISA Kit
7324C PathScan® Phospho-ALK (Tyr1604) Sandwich ELISA Kit
7020C PathScan® Phospho-ALK (Tyr1604) Chemiluminescent Sandwich ELISA Kit
7084C PathScan® Total ALK Chemiluminescent Sandwich ELISA Kit
7322C PathScan® Total ALK Sandwich ELISA Kit
7959C PathScan® Phospho-AMPKα (Thr172) Sandwich ELISA Kit
12850C PathScan® Total Androgen Receptor Sandwich ELISA Kit
7185C PathScan® Phospho-ATF-2 (Thr71) Sandwich ELISA kit
7814C PathScan® Total SQSTM1/p62 Sandwich ELISA Kit
7042C PathScan® Phospho-Axl (panTyr) Sandwich ELISA Kit
7040C PathScan® Total Axl Sandwich ELISA Kit
7182C PathScan® Phospho-Bad (Ser112) Sandwich ELISA Kit
7162C PathScan® Total Bad Sandwich ELISA Kit
7950C PathScan® Phospho-Bcr-Abl (Tyr177) Sandwich ELISA Kit
7190C PathScan® Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit
7870C PathScan® Phospho-Chk1 (Ser317) Sandwich ELISA Kit
7291C PathScan® Total Cox2 Sandwich ELISA Kit
7385C PathScan® Phospho-CREB (Ser133) Sandwich ELISA Kit
7390C PathScan® Total CREB Sandwich ELISA Kit
7918C PathScan® Total Cyclin D1 Sandwich ELISA Kit
7863C PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit
7845C PathScan® Total DDR1 Sandwich ELISA Kit
7189C PathScan® Phospho-EGF Receptor (Tyr845) Sandwich ELISA Kit
7036C PathScan® Phospho-EGF Receptor (Tyr1068) Chemiluminescent Sandwich ELISA Kit
7240C PathScan® Phospho-EGF Receptor (Tyr1068) Sandwich ELISA Kit
7187C PathScan® Phospho-EGF Receptor (Tyr1173) Sandwich ELISA Kit
7250C PathScan® Total EGF Receptor Sandwich ELISA Kit
7911C PathScan® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit
7297C PathScan® Total EGF Receptor Chemiluminescent Sandwich ELISA Kit
7286C PathScan® Phospho-eIF2α (Ser51) Sandwich ELISA Kit
7952C PathScan® Total eIF2α Sandwich ELISA Kit
7980C PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit
7315C PathScan® Phospho-p44 MAPK (Thr202/Tyr204) Sandwich ELISA Kit
7177C PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit
7050C PathScan® Total p44/42 MAPK (Erk1/2) Sandwich ELISA Kit
7689C PathScan® Total Fatty Acid Synthase Sandwich ELISA Kit
12909C PathScan® Phospho-FGF Receptor 1 (panTyr) Sandwich ELISA Kit
12828C PathScan® Total FGF Receptor 1 Sandwich ELISA Kit
7954C PathScan® Phospho-FGFR2 (panTyr) Sandwich ELISA Kit
7939C PathScan® Total FGFR2 Sandwich ELISA Kit
7206C PathScan® Phospho-FLT3 (Tyr591) Sandwich ELISA Kit
7021C PathScan® Phospho-FLT3 (Tyr591) Chemiluminescent Sandwich ELISA Kit
7761C PathScan® Phospho-FLT3 (panTyr) Sandwich ELISA Kit
7202C PathScan® Total FLT3 Sandwich ELISA Kit
7878C PathScan® Total GFP Sandwich ELISA Kit
7311C PathScan® Phospho-GSK-3β (Ser9) Sandwich ELISA Kit
7148C PathScan® Phospho-HER2/ErbB2 (Tyr1221/1222) Sandwich ELISA Kit
7968C PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit
7310C PathScan® Total HER2/ErbB2 Sandwich ELISA Kit
7890C PathScan® Phospho-HER3/ErbB3 (panTyr) Sandwich ELISA Kit
7888C PathScan® Total HER3/ErbB3 Sandwich ELISA kit
7123C PathScan® Mono-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit
7125C PathScan® Tri-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit
7866C PathScan® Tri-Methyl-Histone H3 (Lys27) Sandwich ELISA Kit
7121C PathScan® Acetyl-Histone H3 (Lys9) Sandwich ELISA Kit
7124C PathScan® Di-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit
7868C PathScan® Di-Methyl-Histone H3 (Lys36) Sandwich ELISA Kit
7152C PathScan® Phospho-HSP27 (Ser82) Sandwich ELISA Kit
7295C PathScan® Total HSP27 Sandwich ELISA Kit
7302C PathScan® Phospho-IGF-I Receptor β (Tyr1131) Sandwich ELISA Kit
7355C PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Kit
7360C PathScan® Total IκBα Sandwich ELISA Kit
7073C PathScan® Phospho-IKKα (Ser176/180) Sandwich ELISA Kit
7080C PathScan® Phospho-IKKβ (Ser177/181) Sandwich ELISA Kit
7078C PathScan® Total IKKα Sandwich ELISA Kit
7258C PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Kit
7254C PathScan® Phospho-Insulin Receptor β (Tyr1146) Sandwich ELISA Kit
7326C PathScan® Phospho-Insulin Receptor β (Tyr1345) Sandwich ELISA Kit
7069C PathScan® Total Insulin Receptor β Sandwich ELISA Kit
7082C PathScan® Phospho-Insulin Receptor β (panTyr) Sandwich ELISA Kit
7287C PathScan® Phospho-IRS-1 (Ser307) Sandwich ELISA Kit
7332C PathScan® Phospho-IRS-1 (Ser612) Sandwich ELISA Kit
7133C PathScan® Phospho-IRS-1 (panTyr) Sandwich ELISA Kit
7328C PathScan® Total IRS-1 Sandwich ELISA Kit
7860C PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit
7884C PathScan® Total IRS-2 Sandwich ELISA Kit
7027C PathScan® Phospho-c-Jun (Ser63) Chemiluminescent Sandwich ELISA Kit
7150C PathScan® Total c-Jun Sandwich ELISA Kit II
7231C PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Kit
7197C PathScan® Total c-Kit Sandwich ELISA Kit
7298C PathScan® Phospho-c-Kit (Tyr719) Sandwich ELISA Kit
7946C PathScan® Phospho-p38 MAPK (Thr180/Tyr182) Sandwich ELISA Kit
7251C PathScan® Phospho-MARCKS (Ser152/156) Sandwich ELISA Kit
7175C PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit
7029C PathScan® Phospho-MEK1 (Ser217/221) Chemiluminescent Sandwich ELISA Kit
7241C PathScan® Phospho-Met (Tyr1003) Sandwich ELISA Kit
7227C PathScan® Phospho-Met (Tyr1234/1235) Sandwich ELISA Kit
7896C PathScan® Phospho-Met (Tyr1349) Sandwich ELISA Kit
7333C PathScan® Phospho-Met (panTyr) Sandwich ELISA Kit
7242C PathScan® Total Met Sandwich ELISA Kit
7976C PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit
7978C PathScan® Phospho-mTOR (Ser2481) Sandwich ELISA Kit
7974C PathScan® Total mTOR Sandwich ELISA Kit
7173C PathScan® Phospho-NF-κB p65 (Ser536) Sandwich ELISA Kit
7167C PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Kit
7768C PathScan® Total p27 Kip1 Sandwich ELISA Kit
7365C PathScan® Phospho-p53 (Ser15) Sandwich ELISA Kit
7063C PathScan® Phospho-p70 S6 Kinase (Thr389) Sandwich ELISA Kit
7153C PathScan® Phospho-p70 S6 Kinase (Thr389) Chemiluminescent Sandwich ELISA Kit
7296C PathScan® Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit
7345C PathScan® Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit
7327C PathScan® Phospho-PRAS40 (Thr246) Sandwich ELISA Kit
7331C PathScan® Total PRAS40 Sandwich ELISA Kit
7285C PathScan® Phospho-PTEN (Ser380) Sandwich ELISA Kit
7882C PathScan® Total PTEN Sandwich ELISA Kit
12321C PathScan® Phospho-Ret (panTyr) Chemiluminescent Sandwich ELISA Kit
7034C PathScan® Phospho-Ret (panTyr) Sandwich ELISA Kit
7032C PathScan® Total Ret Sandwich ELISA Kit
7093C PathScan® Phospho-ROS1 (panTyr) Sandwich ELISA Kit
7091C PathScan® Total ROS1 Sandwich ELISA Kit
7316C PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Chemiluminescent Sandwich ELISA Kit
7205C PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Kit
7337C PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit
7225C PathScan® Total S6 Ribosomal Protein Sandwich ELISA Kit
7849C PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Chemiluminescent Sandwich ELISA Kit
7325C PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Kit
7330C PathScan® Total SAPK/JNK Sandwich ELISA Kit
7348C PathScan® Phospho-Smad2 (Ser465/467) Sandwich ELISA Kit
7244C PathScan® Total Smad2 Sandwich ELISA Kit
12000C PathScan® Total Smad2/3 Sandwich ELISA Kit
7953C PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit
7984C PathScan® Total Src Sandwich ELISA Kit
7234C PathScan® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit
7149C PathScan® Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit
7300C PathScan® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit
8026C PathScan® Phospho-Stat3 (Ser727) Chemiluminescent Sandwich ELISA Kit
7305C PathScan® Total Stat3 Sandwich ELISA Kit
7995C PathScan® Phospho-Stat3 (Ser727) Sandwich ELISA Kit
7113C PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Kit
7275C PathScan® Phospho-Stat6 (Tyr641) Sandwich ELISA Kit
7267C PathScan® Total Stat6 Sandwich ELISA Kit
7970C PathScan® Phospho-Syk (Tyr525/526) Sandwich ELISA Kit
7928C PathScan® Phospho-Syk (panTyr) Sandwich ELISA Kit
7210C PathScan® Phospho-TrkA (Tyr490) Sandwich ELISA Kit
7212C PathScan® Phospho-TrkA (Tyr674/675) Sandwich ELISA Kit
7208C PathScan® Total TrkA Sandwich ELISA Kit
7111C PathScan® Phospho-TrkB (Tyr516) Sandwich ELISA Kit
7087C PathScan® Phospho-TrkB (panTyr) Chemiluminescent Sandwich ELISA Kit
7108C PathScan® Phospho-TrkB (panTyr) Sandwich ELISA Kit
7340C PathScan® Total VEGFR-2 Sandwich ELISA Kit
7335C PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit
7172C PathScan® Total Zap-70 Sandwich ELISA Kit