| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| AceCS1 (D19C6) Rabbit mAb | 3658 | 20 µl | 78 kDa | Rabbit IgG |
| Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb | 11818 | 20 µl | 280 kDa | Rabbit IgG |
| Acetyl-CoA Carboxylase (C83B10) Rabbit mAb | 3676 | 20 µl | 280 kDa | Rabbit IgG |
| ATP-Citrate Lyase Antibody | 4332 | 20 µl | 125 kDa | Rabbit |
| Phospho-ATP-Citrate Lyase (Ser455) Antibody | 4331 | 20 µl | 125 kDa | Rabbit |
| Fatty Acid Synthase (C20G5) Rabbit mAb | 3180 | 20 µl | 273 kDa | Rabbit IgG |
| Lipin 1 (D2W9G) Rabbit mAb | 14906 | 20 µl | 130 kDa | Rabbit IgG |
| ACSL1 (D2H5) Rabbit mAb | 9189 | 20 µl | 78 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Fatty Acid and Lipid Metabolism Antibody Sampler Kit provides an economical means to evaluate key proteins involved in fatty acid and lipid metabolism. This kit includes enough primary antibody to perform two western miniblot experiments with each primary antibody.
Storage
Background
The processes of fatty acid and lipid metabolism are vital for cellular nutrient and energy maintenance. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1,2). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (4). ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for glucogenesis (5). Phosphorylation of ACL at Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (6). Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA (7). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (8-10). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in the liver and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (11).
- Ikeda, Y. et al. (2001) J Biol Chem 276, 34259-69.
- Luong, A. et al. (2000) J Biol Chem 275, 26458-66.
- Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
- Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
- Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33.
- Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79.
- Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
- Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
- Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
- Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
- Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.