| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Desmin (D93F5) XP® Rabbit mAb | 5332 | 20 µl | 53 kDa | Rabbit IgG |
| Keratin 17 (D73C7) Rabbit mAb | 4543 | 20 µl | 48 kDa | Rabbit IgG |
| Pan-Keratin (C11) Mouse mAb | 4545 | 20 µl | 46-58 kDa | Mouse IgG1 |
| β-Tubulin (9F3) Rabbit mAb | 2128 | 20 µl | 55 kDa | Rabbit IgG |
| Vimentin (D21H3) XP® Rabbit mAb | 5741 | 20 µl | 57 kDa | Rabbit IgG |
| β-Actin (D6A8) Rabbit mAb | 8457 | 20 µl | 45 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform two western blots per primary antibody.
Storage
Background
The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6).
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- Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
- Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
- Moll, R. et al. (1982) Cell 31, 11-24.
- Leader, M. et al. (1987) Histopathology 11, 63-72.
- Li, Z. et al. (1996) Dev Biol 175, 362-6.
- Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
Background References
Trademarks and Patents
Limited Uses
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