Bio-Plex

Bio-Plex nine-plex phosphoprotein assay. Following treatment of HeLa cells with TNF-alpha or IFN, the phosphorylation status of GSK3-alpha/ beta (Ser21/9), Akt (Ser473), MEK2 (Ser221), ATF-2 (Thr69), Stat3 (Tyr705), JNK (Thr183/Tyr185), p38MAPK (Thr180/Tyr182), IkB-alpha (Ser32/36), and p44/42 (Thr202/Tyr204) were assessed using a cocktail of bead-conjugated capture antibodies and biotinylated detection antibodies. With the addition of streptavidin-PE, mean fluorescence intensity (MFI) for each bead is quantified in proportion to the levels of each phosphorylated protein.

Cell Signaling Technology and Bio-Rad have partnered to develop multiplex phosphoprotein assays on Bio-Rad’s Bio-Plex® Suspension Array System. CST is developing highly specific antibodies for exclusive use in Bio-Plex phosphoprotein assays. The Bio-Plex platform enables profiling of multiple signaling proteins in a single well of a 96-well plate. Singleplex and multiplex total- and phosphoprotein assays are available from Bio-Rad as off-the-shelf and customizable x-Plex assays (see www.bio-rad.com/products/phosphoproteins for details).

Bio-Plex Phosphoprotein Assays

Product Name	Catalog #
Bio-Plex Phospho-Akt (Ser473) Assay	171-V21075
Bio-Plex Total ATF-2 Assay	171-V31620
Bio-Plex Phospho-ATF-2 (Thr71) Assay	171-V21620
Bio-Plex Phospho-EGFR (Tyr) Assay	171-V23120
Bio-Plex Phospho-Erk1 (Thr202/Tyr204) Assay	171-V21938
Bio-Plex Total Erk2 Assay	171-V30438
Bio-Plex Phospho-Erk2 (Thr185/Tyr187) Assay	171-V20438
Bio-Plex Phospho-Erk1/2 (Thr202/Tyr204) Assay	171-V22238
Bio-Plex Phospho-GSK3α/β (Ser21/9) Assay	171-V23318
Bio-Plex Total IκB-α Assay	171-V30758
Bio-Plex Phospho-IκB-α (Ser32/36) Assay	171-V20758
Bio-Plex Total JNK Assay	171-V31034
Bio-Plex Phospho-JNK (Thr183/Tyr185) Assay	171-V21034
Bio-Plex Total p38 MAPK Assay	171-V31336
Bio-Plex Phospho-p38 MAPK (Thr180/Tyr182) Assay	171-V21336
Bio-Plex Phospho-STAT3 (Tyr705) Assay	171-V22552