PathScan® ELISA
The scientists at Cell Signaling Technology® develop the highest quality research tools that are robust, sensitive and specific. Our line of ELISA products provides researchers with a wide selection of assays measuring important cellular signaling molecules. In-house development, production and validation of these kits ensure the highest possible product quality and support. Technical support is provided by the same scientists that produce the ELISA products.
ELISA Product Line
- PathScan® Sandwich ELISA Kits – contain all necessary components for detection of endogenous levels of key signaling molecules. Matched phospho and total protein ELISA kits are available for many targets. Colorimetric and chemiluminescent detection options are available.
- PathScan® Antibody Array Kits – are slide-based ELISA assays, designed to investigate signal transduction occurring through receptor tyrosine kinases and pathway key nodes in a multiplex format.
- PathScan® Sandwich ELISA Antibody Pairs – provide scientists with an economical alternative to our complete ELISA kits.
- PathScan® Multi-Target ELISA Kits – examine several important and well-characterized signaling events in a single assay.
- PathScan® ELISA Control Cell Extracts – provide the appropriate positive and negative controls, and also allow the standardization of signal obtained from different plates.
- SignalKine™ Human TNF-α Sandwich ELISA Kit #7289 – allows researchers to detect hTNF-α in serum, plasma, and cell culture supernatants.
- Custom ELISA products – allow researchers the option of different detection methods and plate formats. Convenient bulk packaging is available upon request.
Companion Products
- PathScan® ELISA Control Cell Extracts
- PathScan® Sandwich ELISA Lysis Buffer (1X) #7018
- BSA #9998
- Cell Lysis Buffer (10X) #9803
- Phosphate Buffered Saline (PBS-20X) #9808
- Phosphate Buffered Saline with Tween 20 (PBST-20X) #9809
- STOP Solution #7002
- TMB Substrate #7004
PathScan® Sandwich ELISA Products
| Target | Colorimetric Sandwich ELISA Kits | Chemiluminescent Sandwich ELISA Kits | Antibody Pairs | Multi-Target Kits | ELISA Control Cell Extract |
|---|---|---|---|---|---|
| Target | Colorimetric Sandwich ELISA Kits | Chemiluminescent Sandwich ELISA Kits | Antibody Pairs | Multi-Target Kits | ELISA Control Cell Extract |
| Phospho-4E-BP1 (Thr37/Thr46) | 7216 | 7854 | |||
| 4E-BP1 | 7179 | ||||
| Phospho-Acetyl CoA Carboxylase (Ser79) | 7986 | ||||
| Acetyl-CoA Carboxylase | 7996 | ||||
| β-Actin | 7880 | 7881 | |||
| Phospho-AMPKα (Thr172) | 7959 | 7955 | |||
| Phospho-Akt (Thr308) | 7252 | 7135 | 7144 | Cell Growth 7239 | Cell Extract II 7989 |
| Phospho-Akt1 (Ser473) | 7160 | 7134 | 7143 | Cell Growth 7239 Signaling Nodes 7272 | Cell Extract I 7988 |
| Akt1 | 7170 | 7132 | 7142 | Cell Growth 7239 Signaling Nodes 7272 | Cell Extract II 7989 |
| Phospho-Akt2 (Ser474) | 7048 | ||||
| Phospho-Akt2 (Ser474) (mouse preferred) | 7932 | ||||
| Akt2 | 7046 | ||||
| Phospho-Akt3 (Ser472) (mouse preferred) | 7942 | ||||
| Akt3 (mouse preferred) | 7934 | ||||
| Phospho-ALK (Tyr1586) | 7159 | ||||
| Phospho-ALK (Tyr1604) | 7324 | 7020 | |||
| ALK | 7322 | 7084 | |||
| Phospho-ATF-2 (Thr71) | 7185 | Cell Extract II 7989 | |||
| Phospho-Aurora A (Thr288) | 7114 | 7115 | |||
| Aurora A | 7116 | 7117 | |||
| Axl (pan p-Tyr) | 7042 | ||||
| Axl | 7040 | ||||
| Phospho-Bad (Ser112) | 7182 | 7842 | Apoptosis 7105 | Cell Extract II 7989 | |
| Bad | 7162 | 7840 | Apoptosis 7105 | Cell Extract I 7988 | |
| β-Catenin | 7308 | 7309 | |||
| E-Cadherin | 7886 | 7887 | |||
| Cleaved Caspase-3 (Asp175) | 7190 | Apoptosis 7105 | |||
| Phospho-cdc2 (Tyr15) | 7176 | 7838 | |||
| Phospho-Chk1 (Ser317) | 7870 | Cell Extract II 7989 | |||
| Chk1 | 7872 | 7873 | |||
| Phosho-Chk2 (Thr68) | 7037 | ||||
| Chk2 | 7045 | 7090 | |||
| Phospho-c-Jun (Ser63) | 7145 | 7027 | 7141 | ||
| c-Jun | 7150 | 7028 | 7314 | ||
| Cox2 | 7291 | ||||
| Phospho-CREB (Ser133) | 7385 | ||||
| CREB | 7390 | ||||
| Phospho-DDR1 (pan p-Tyr) | 7863 | ||||
| DDR1 | 7845 | ||||
| Phospho-EGFR (Tyr845) | 7189 | ||||
| Phospho-EGFR (Tyr1068) | 7240 | 7036 | |||
| Phospho-EGFR (Tyr1173) | 7187 | ||||
| EGFR (E746-A750del Specific) | 8950 | ||||
| EGFR (L858R Mutant Specific) | 8951 | ||||
| EGFR | 7250 | ||||
| Phospho-eIF2α (Ser51) | 7948 | Cell Extract I 7988 | |||
| eIF2α | 7952 | Cell Extract I 7988 | |||
| Phospho-eIF4E (Ser209) | 7938 | ||||
| eIF4E | 7940 | ||||
| Phospho-eNOS (Ser1177) | 7980 | ||||
| Phospho-Erk1 (Thr202/Tyr204) | 7315 | 7278 | |||
| Phospho-Erk1/2 (Thr202/Tyr204) | 7177 | 7246 | Cell Growth 7239 MAP Kinase 7274 | ||
| Erk 1/2 | 7050 | ||||
| Fatty Acid Synthase | 7689 | ||||
| Phospho-FGFR2 (panTyr) | 7954 | ||||
| FGFR2 | 7939 | ||||
| Phospho-Flt3 (pan p-Tyr) | 7761 | ||||
| Phospho-Flt3 (Tyr591) | 7206 | 7021 | |||
| Flt3 | 7202 | ||||
| GFP | 7878 | 7879 | |||
| Phospho-GSK-3β (Ser9) | 7311 | ||||
| GSK-3β | 7265 | ||||
| Phospho-HER2 (pan p-Tyr) | 7968 | ||||
| Phospho-HER2 (Tyr1221/1222) | 7148 | 7817 | |||
| HER2 | 7310 | ||||
| Phospho-HER3 (pan p-Tyr) | 7890 | ||||
| HER3 | 7888 | ||||
| Acetyl-Histone H2A | 7233 | ||||
| Acetyl-Histone H2B | 7178 | ||||
| Acetyl-Histone H2B (Lys5) | 7218 | ||||
| Acetyl-Histone H2B (Lys20) | 7222 | ||||
| Acetyl-Histone H3 | 7232 | 7209 | |||
| Acetylated Histone H3 (Lys9) | 7121 | ||||
| Acetylated Histone H3 (Lys18) | 7122 | ||||
| Mono-Methyl Histone H3 (Lys4) | 7123 | ||||
| Di-Methyl-Histone H3 (Lys4) | 7124 | ||||
| Tri-Methyl Histone H3 (Lys4) | 7125 | ||||
| Pan-Methyl Histone H3 (Lys9) | 7864 | ||||
| Di-Methyl Histone H3 (Lys9) | 7862 | ||||
| Tri-Methyl Histone H3 (Lys27) | 7866 | ||||
| Di-Methyl Histone H3 (Lys36) | 7868 | ||||
| Phospho-Histone H3 (Ser10) | 7155 | 7303 | 7207 | ||
| Histone H3 | 7253 | ||||
| Acetyl-Histone H4 | 7238 | ||||
| Acetyl-Histone H4 (Lys8) | 7224 | ||||
| Acetyl-Histone H4 (Lys12) | 7228 | ||||
| Phospho-HSP27 (Ser78) | 7290 | ||||
| Phospho-HSP27 (Ser82) | 7152 | ||||
| HSP27 | 7295 | ||||
| Phospho-IGF1 Receptor (Tyr1131) | 7302 | 7820 | |||
| Phospho-IκB-α (Ser32) | 7355 | 7343 | Inflammation 7276 | ||
| IκB-α | 7360 | 7831 | |||
| Phospho-IKKα (Ser176/180) | 7073 | ||||
| IKKα | 7078 | ||||
| Phospho-IKKβ (Ser177/181) | 7080 | ||||
| iNOS | 7097 | ||||
| Phospho-Insulin Receptor β (Tyr1146) | 7254 | 7827 | |||
| Phospho-Insulin Receptor β (Tyr1150/1151) | 7258 | 7828 | |||
| Phospho-Insulin Receptor β (Tyr1345) | 7326 | 7823 | |||
| Phospho-Insulin Receptor β (pan p-Tyr) | 7082 | ||||
| Insulin Receptor β | 7069 | ||||
| Phospho-IRS-1 (pan p-Tyr) | 7133 | 7347 | |||
| Phospho-IRS-1 (Ser302) | 7283 | 7284 | |||
| Phospho-IRS-1 (Ser307) | 7287 | 7288 | |||
| Phospho-IRS-1 (Ser612) | 7332 | ||||
| IRS-1 | 7328 | ||||
| Phospho-IRS-2 (pan p-Tyr) | 7860 | 7861 | |||
| IRS-2 | 7884 | 7885 | |||
| Phospho-c-Kit (pan p-Tyr) | 7231 | 7294 | |||
| Phospho-c-Kit (Tyr719) | 7298 | 7299 | |||
| c-Kit | 7197 | ||||
| Phospho-LAT (Tyr191) | 7936 | 7937 | |||
| Phospho-Lck (Tyr505) | 7941 | 7993 | |||
| Phospho-MARCKS (Ser152/156) | 7251 | ||||
| MARCKS | 7188 | ||||
| Phospho-MEK1 (Ser217/221) | 7175 | 7029 | 7211 | Signaling Nodes 7272 MAP Kinase 7274 | Cell Extract I 7988 |
| MEK1 | 7165 | 7030 | 7215 | MAP Kinase 7274 | |
| Phospho-Met (pan p-Tyr) | 7333 | 7334 | |||
| Phospho-Met (Tyr1003) | 7241 | ||||
| Phospho-Met (Tyr1234/1235) | 7227 | 7229 | |||
| Phospho-Met (Tyr1349) | 7896 | ||||
| Met | 7242 | ||||
| Phospho-mTOR (Ser2448) | 7976 | ||||
| Phospho-mTOR (Ser2481) | 7978 | ||||
| mTOR | 7974 | ||||
| Phospho-NF-κB p65 (Ser536) | 7173 | 7834 | Inflammation 7276 Signaling Nodes 7272 | ||
| NF-κB p65 | 7174 | 7836 | Inflammation 7276 | Cell Extract I 7988 | |
| Cleaved Notch1 (Val1744) | 7194 | ||||
| Notch1 | 7245 | ||||
| p21 WAF1/CIP | 7167 | 7856 | |||
| Phospho-p38 (Thr180/Tyr182) | 7946 | MAP Kinase 7274 Inflammation 7276 Signaling Nodes 7272 | |||
| Acetyl-p53 | 7236 | 7848 | |||
| Phospho-p53 (Ser15) | 7365 | 7846 | Apoptosis 7105 | ||
| p53 | 7370 | 7844 | Apoptosis 7105 | ||
| Phospho-p70 S6 Kinase (Thr389) | 7063 | 7153 | 7053 | ||
| p70 S6 Kinase | 7038 | 7039 | |||
| Phospho-p90 Rsk1 (Ser380) | 7965 | ||||
| p90 Rsk1 | 7966 | ||||
| Cleaved PARP (Asp214) | 7262 | 7858 | Apoptosis 7105 | ||
| Phospho-PDGFR α/β (pan p-Tyr) | 7235 | 7307 | |||
| Phospho-PDGFR α (Tyr849) | 7296 | 7317 | |||
| PDGFR α | 7318 | 7264 | |||
| Phospho-PDGFR β (Tyr751) | 7345 | 7826 | |||
| Phospho-PRAS40 (Thr246) | 7327 | ||||
| PRAS40 | 7331 | ||||
| Phospho-PTEN (Ser380) | 7285 | ||||
| PTEN | 7882 | 7883 | |||
| Phospho-Ret (pan p-Tyr) | 7034 | ||||
| Ret | 7032 | ||||
| Phospho-Ros (pan p-Tyr) | 7093 | ||||
| Ros | 7091 | ||||
| Phospho-S6 Ribosomal Protein (Ser235/236) | 7205 | 7316 | 7201 | Cell Growth 7239 | Cell Extract I 7988 |
| S6 Ribosomal Protein | 7225 | 7337 | 7203 | Cell Growth 7239 | Cell Extract I 7988 |
| Phospho-SAPK/JNK1/2/3 (Thr183/Tyr185) | 7325 | 7849 | 7217 | MAP Kinase 7274 Inflammation 7276 | Cell Extract II 7989 |
| Phospho-Src (Tyr416) | 7953 | 7963 | |||
| Src | 7984 | 7992 | |||
| SAPK/JNK1/2/3 | 7330 | 7869 | 7219 | MAP Kinase 7274 | Cell Extract II 7989 |
| Phospho-Smad2 (Ser465/467) | 7348 | ||||
| Smad2 | 7244 | ||||
| Phospho-Stat1 (Tyr701) | 7234 | ||||
| Phospho-Stat3 (Tyr705) | 7300 | 7149 | 7146 | Signaling Nodes 7272 Inflammation 7276 | |
| Phospho-Stat3 (Ser727) | 7995 | 8026 | |||
| Stat3 | 7305 | ||||
| Phospho-Stat5 (Tyr694) | 7113 | 7281 | |||
| Phospho-Stat6 (Tyr641) | 7304 | ||||
| Phospho-Survivin (Thr34) | 7193 | ||||
| Survivin | 7169 | ||||
| Phospho-Syk (pan p-Tyr) | 7928 | 7929 | |||
| Phospho-Syk (Tyr525/526) | 7970 | ||||
| Phospho-TrkA (Tyr490) | 7210 | ||||
| Phospho-TrkA (Tyr674/675) | 7212 | ||||
| TrkA | 7208 | ||||
| Phospho-TrkB (pan p-Tyr) | 7108 | ||||
| Phospho-TrkB (Tyr516) | 7111 | ||||
| Phospho-TrkB (Tyr706/707) | 7118 | ||||
| TrkB | 7106 | ||||
| α-Tubulin | 7944 | 7945 | Cell Extract II 7989 | ||
| Acetyl-Tubulin | 7204 | ||||
| Phospho-VEGFR-2 (Tyr1175) | 7335 | 7824 | |||
| VEGFR-2 | 7340 | 7825 | |||
| Phospho-Zap-70 (Tyr319) | 7171 | 7852 | |||
| Zap-70 | 7172 | 7850 |
