Cell Signaling Technology

High Content Screening

High Content Screening

Localization of phosphorylated p44/42 in NIH/3T3 cells treated with PMA and examined on the IN Cell Analyzer 1000 using the Phospho-p44/42 (Thr202/Tyr204) (E10) Monoclonal Antibody #9106 (top). Dose dependent nuclear localization of active p44/42 in PMA-treated cells. Quantification was done using the Nuclear Tracking Analysis Module on the IN Cell Analyzer 1000 (bottom) (data courtesy GE Healthcare).

High content screening (HCS) enables cell-based assays of kinase activation, protein phosphorylation, cell morphology, and protein localization. HCS is based on the fixation and staining of adherent cells with high affinity/specificity antibodies and complex image acquisition/analysis software to gather information about the characteristics of single cells. Several imaging platforms have been commercialized including those available from GE Healthcare, Acumen, CompuCyte, and others.

Available Products

No. Name
9661 Cleaved Caspase-3 (Asp175) Antibody
9191 Phospho-CREB (Ser133) Antibody
9186 Phospho-Elk-1 (Ser383) (2B1) Mouse mAb
9106 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes