High Content Screening
Localization of phosphorylated p44/42 in NIH/3T3 cells treated with PMA and examined on the IN Cell Analyzer 1000 using the Phospho-p44/42 (Thr202/Tyr204) (E10) Monoclonal Antibody #9106 (top). Dose dependent nuclear localization of active p44/42 in PMA-treated cells. Quantification was done using the Nuclear Tracking Analysis Module on the IN Cell Analyzer 1000 (bottom) (data courtesy GE Healthcare).
High content screening (HCS) enables cell-based assays of kinase activation, protein phosphorylation, cell morphology, and protein localization. HCS is based on the fixation and staining of adherent cells with high affinity/specificity antibodies and complex image acquisition/analysis software to gather information about the characteristics of single cells. Several imaging platforms have been commercialized including those available from GE Healthcare, Acumen, CompuCyte, and others.
Available Products
| No. | Name |
|---|---|
| 9661 | Cleaved Caspase-3 (Asp175) Antibody |
| 9191 | Phospho-CREB (Ser133) Antibody |
| 9186 | Phospho-Elk-1 (Ser383) (2B1) Mouse mAb |
| 9106 | Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb |
