LANCE
LANCE FRET between a Eu chelate and the APC acceptor occurs following phosphorylation of a substrate peptide by a kinase.
LANCE (PerkinElmer Life Sciences) is based on the proximity of a donor label (europium chelate) and an acceptor label (Allophycocyanin) that have been brought together by a specific binding reaction. The energy of the europium chelate is transferred by nonradiative fluorescence resonance energy transfer (FRET) to an acceptor within a short distance. Fluorescent lanthanide chelates with long excited state lifetimes are used to avoid interference caused by short-lived emission from acceptor molecules excited directly rather than by energy transfer. LANCE TR-FRET has been widely used in kinase, nuclear receptor and protein binding assays.
Nearly all of Cell Signaling Technology antibodies are available in a formulation without BSA or glycerol for conjugation to lanthanide chelates. Please contact us at drugdiscovery@cellsignal.com for details.
Available Products
| No. | Name |
|---|---|
| 9391 | Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) |
