In-Cell Western™ (LI-COR^®)

In-Cell Western™ is a simple and cost effective cell-based assay that permits the quantification of intracellular signaling. This assay involves seeding cells in microtiter plates followed by fixation/permeabilization and subsequent labeling with activation state-specific or control antibodies and infrared-conjugated secondary antibodies. In collaboration with LI-COR^® Biosciences (www.licor.com), CST has validated many antibodies for use in the In-Cell Western™ format using the Odyssey^® Infrared Imaging System.

For the most up-to-date list of ICW-validated antibodies, information on other IF-validated antibodies appropriate for ICW, or assistance with your assay design, please contact ICW@cellsignal.com.

Odyssey^®, LI-COR^® and In-Cell Western™ are registered trademarks or trademarks of LI-COR Biosciences, Inc.).

Image representing well-level fluorescent signaling intensity of antibodies in the PathScan^® Multi-Target Automated IF PI3K/Akt Kit measured using the LI-COR Odyssey^® Infrared Imaging System. C2C12 mouse myoblast cells were grown in 96-well plates, treated with either insulin (15 min) or the PI3K inhibitor LY294002 (3 hr), fixed, and labeled with antibodies in the PathScan^® Multi-Target Automated IF PI3K/Akt Kit. Responses of each antibody were measured as mean fluorescence intensity per well.