Nonradioactive Kinase Assay Kits
Nonradioactive kinase assay kits provide all the reagent necessary to measure the kinase activity within a cell. The process involves selective IP of the kinase using an immobilized antibody and incubation of the IP pellets in kinase buffer containing the fusion protein substrate and ATP. The phosphorylated substrate is then detected by Western blotting using a phospho-specific antibody. These kits are useful for determining changes in kinase activity after treatment of cells with novel kinase inhibitors.
| No. | Kinase Assay Kit (Nonradioactive) | Reactivity |
|---|---|---|
| 9840 | Akt | H, M, R |
| 9820 | p38 MAPK | H, M |
| 9800 | p44/42 MAPK | H, M, R |
Nonradioactive Kinase Assay Kit Overview
Step 1: Selective IP of Akt using Immobilized Akt Antibody.
a) Add immobilized Antibody. b) IP Kinase from cell extracts using Immobilized Akt Antibody.
Step 2: Incubate IP pellets in Kinase Buffer containing Recombinant fusion protein and cold ATP.
Step 3: Detect Recombinant fusion protein phosphorylation using phospho specific antibodies by Western blotting and chemiluminescent detection.
Publications
Kit 9840
- Frischknecht K, Greutert H, Weisshaupt C, Kaspar M, Yang Z, Luscher TF, Carrel TP, Tanner FC (2006) Different vascular smooth muscle cell apoptosis in the human internal mammary artery and the saphenous vein. Implications for bypass graft disease. J. Vasc. Res. 43(4), 338–46.
- Freude S, Plum L, Schnitker J, Leeser U, Udelhoven M, Krone W, Bruning JC, Schubert M (2005) Peripheral hyperinsulinemia promotes tau phosphorylation in vivo. Diabetes 54(12), 3343–8.
Kit 9820
- Milano G, Morel S, Bonny C, Samaja M, von Segesser LK, Nicod P, Vassalli G (2007) A peptide inhibitor of c-Jun NH2-terminal kinase reduces myocardial ischemia-reperfusion injury and infarct size in vivo. Am. J. Physiol. Heart Circ. Physiol. 292(4), H1828–35.
- Schito ML, Demidov ON, Saito S, Ashwell JD, Appella E (2006) Wip1 phosphatase-deficient mice exhibit defective T cell maturation due to sustained p53 activation. J. Immunol. 176(8), 4818–25.
