PathScan® Chemiluminescent Sandwich ELISA Kits
New PathScan® Chemiluminescent Sandwich ELISA Kits have a wider dynamic range and greater sensitivity than conventional chromogenic detection systems. These kits are offered in low volume microplates which retain high signal and sensitivity while using smaller samples sizes.
- In-house development, validation and optimization ensure the highest ELISA kit quality.
- Chemiluminescent detection system offers the broadest dynamic range and greatest assay sensitivity.
- Low volume microplates show increased signal and sensitivity while using half the sample size of the traditional colorimetric ELISA.
- Technical support provided by the same scientists that develop and produce the kits.
Phospho-Akt1 (Ser473)
The graph shows levels of Phospho-Akt1 (Ser473) in PDGF-treated and untreated NIH/3T3 cells. Signal intensity, determined by chemiluminescence readout is given in RLUs (Relative Light Units). The signal is proportional to the amount of protein lysate added.
The inset graph shows high sensitivity and a linear response (PDGF-treated), even at the low end of protein concentration.
Figure 1: Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF (50 ng/ml) and lysed after incubation at 37ºC for 20 minutes. Graph insert corresponds to the shaded area.
