Protein Substrates

Cell Signaling Technology (CST) offers a variety of protein kinase substrates useful for detecting the activity of protein kinases involved in cell cycle and signal transduction pathways. All of the substrates are cloned and overexpressed in E. coli and purified by affinity chromatography to assure the absence of contaminating eukaryotic kinases and phosphatases.

Quality Assurance

Protein kinase substrates are examined for purity by SDS-PAGE analysis and quality control tested by Western blotting using our complementary antibodies. Each substrate is also tested for its ability to be phosphorylated by its upstream kinase. This is confirmed by Western blotting with the appropriate CST phospho-specific antibody.

Protein Kinase Substrates		Residues	Kinase	Phosphorylation Sites	MW
6082	Inactive p42 MAP Kinase (Erk2)	full length	MEK1/2	Thr183, Tyr185	42 kDa
9184	Elk-1 Fusion Protein	307–428	MAPK/Erk, SAPK/JNK	Ser383	41 kDa
6093	c-Jun Fusion Protein	1–89	SAPK/JNK	Ser63, Ser73	37 kDa
9811	c-Jun Fusion Protein Beads	1–89	SAPK/JNK	Ser63, Ser73
9224	ATF-2 Fusion Protein	19–96	p38 MAPK, SAPK/JNK	Thr69, Thr71	34 kDa
9237	GSK-3 Fusion Protein	18–24	Akt1	Ser21	27 kDa