Cell Signaling Technology (CST) offers a variety of protein kinase substrates useful for detecting the activity of protein kinases involved in cell cycle and signal transduction pathways. All of the substrates are cloned and overexpressed in E. coli and purified by affinity chromatography to assure the absence of contaminating eukaryotic kinases and phosphatases.
Protein kinase substrates are examined for purity by SDS-PAGE analysis and quality control tested by Western blotting using our companion antibodies. Each substrate is also tested for its ability to be phosphorylated by its upstream kinase. This is confirmed by Western blotting with the appropriate CST phospho-specific antibody.
|Protein Kinase Substrates||Residues||Kinase||Phosphorylation Sites||MW|
|9224||ATF-2 Fusion Protein||19–96||p38 MAPK, SAPK/JNK||Thr69, Thr71||34 kDa|
|9184||Elk-1 Fusion Protein||307–428||MAPK/Erk, SAPK/JNK||Ser383||41 kDa|
|9237||GSK-3 Fusion Protein||18–24||Akt1||Ser21||27 kDa|
|6093||c-Jun Fusion Protein||1–89||SAPK/JNK||Ser63, Ser73||37 kDa|
|9811||c-Jun Fusion Protein Beads||1–89||SAPK/JNK||Ser63, Ser73|