Stem Cell Markers
Stem cell biology, the study of mechanisms causing the undifferentiated embryonic or adult stem cells to differentiate, is one of the fastest growing areas in biology today. Cell Signaling Technology's goal is to accelerate stem cell research by developing the highest quality, most rigorously validated antibodies for the study of stem cell markers, epigenetic regulation and stem cell signaling.
Ternary complex of the DNA binding domains of Oct1 (red) and Sox2 (yellow) bound to cognate DNA (in this case a region of the HoxB1 regulatory element).
| New | No. | Antibody | Application | Reactivity | Lineage | Stage | Description |
|---|---|---|---|---|---|---|---|
| new | 3663 | CD133 (C24B9) Rabbit mAb | W, IP, IHC-P, F | H | early embryo, mesoderm, ectoderm | blastocyst, hematopoietic stem cells, neuronal stem cells | Five-transmembrane glycosylated cell-surface protein initially described as a hematopoietic stem cell marker. Also expressed in and used to identify neuronal, cancer and embryonic stem cells. |
| new | 2750 | Oct-4 Antibody | W, IHC-P, IF-IC, F | H | early embryo | blastocyst | Transcription factor expressed in undifferentiated pluripotent embryonic stem cells and germ cells during normal development. Together with Sox2 and Nanog, is necessary for the maintenance of pluripotent potential. |
| new | 2273 | SCF Antibody | W, IHC-P | H | early embryo | blastocyst | Mediates biological effects by binding to and activating c-kit. Promotes proliferation of embryonic stem cells and hematopoetic stem cells. Essential for hematopoiesis, melanogenesis and fertility. |
| new | 2748 | Sox2 Antibody | W, IP | H, M | early embryo | blastocyst | Transcription factor expressed in undifferentiated pluripotent embryonic stem cells and germ cells during development. Together with Oct-4 and Nanog, is necessary for the maintenance of pluripotent potential. |
| new | 3670 | GFAP (GA5) Mouse mAb | W, IP, IHC-P, IF-F | H, M, R | ectoderm | neural stem cell/ ectodermal progenitor | Marker protein specifically expressed in astrocytes. |
| new | 4706 | Integrin β1 Antibody | W | H, M, R, Mk, Hm | ectoderm | bulge stem cell/ mesenchymal stem cell | Cell-adhesion molecule important in cell adhesion and migration. Marker expressed on mesenchymal stem cells, bulge stem cells and during development of the liver. |
| new | 2274 | MELK Antibody | W, IP | H, M | ectoderm | neural stem cell | Implicated in stem cell renewal, cell cycle progression and pre-mRNA splicing. Marker for neural progenitors. |
| new | 2154 | Musashi Antibody | W, IF-F | H, M, R | ectoderm | neural stem cell | RNA-binding protein that regulates the translation of target mRNA during asymmetric cell division of ectodermal precursor cells. Marker for proliferating neural precursor cells. Expressed in epithelial stem cells including intestinal and mammary gland stem cells. |
| new | 2137 | AFP Antibody | W, IP | H, M | endoderm | hematopoietic stem cell | Glycoprotein found in serum of mammalian fetal liver, yolk sac and GI tract. Expressed during development of the primitive endoderm. Marker for endodermal differentiation from pluripotent stem cells. |
| new | 2158 | VE-Cadherin Antibody | W, IP, IF-IC | H, B | mesoderm | hematopoietic stem cell | Adhesion molecule expressed in embryonic (not adult) hematopoietic stem cells. Also expressed in vascular smooth muscle cells. |
| new | 3569 | CD34 (ICO115) Mouse mAb | IHC-P, F | H | mesoderm | hematopoietic stem cell | Transmembrane glycophosphoprotein expressed in hematopoietic skin cells, vascular endothelium and some fibroblasts. |
| new | 4115 | CDCP1 Antibody | W, IP, IF-IHC | H | mesoderm | hematopoietic stem cell | Multidomain membrane glycoprotein highly expressed in some human cancer cells and in both typical and atypical (cancerous) colon. May be epigenetically regulated. Putative hematopoetic stem cell marker. |
| new | 3392 | c-Kit Antibody | W, IP | H, M | mesoderm | hematopoietic stem cell | Receptor tyrosine kinase that plays a critical role in cell growth and proliferation in hematopoietic stem cells, mast cells, melanocytes and germ cells. Binding of its ligand (SCF) to c-kit initiates proliferation and differentiation of hematopoietic progenitor cells. Hematopoietic stem cell marker. |
| new | 3308 | c-Kit (Ab81) Mouse mAb | W, IP, F | H | mesoderm | hematopoietic stem cell | |
| new | 3310 | c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate) | IF-IC, F | H | mesoderm | hematopoietic stem cell | |
| new | 2266 | PU.1 Antibody | W, IP, IHC-P, IF-IC, F | H, M, (Mk) | mesoderm | hematopoietic stem cell | Member of the Ets family of transcription factors that plays a pivotal role in the differentiation of myeloid cells and lymphocytes. Expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells and megakaryocytes. Critical for both determination of hematopoietic cell lineage and regulation of differentiation vs. stem cell proliferation. |
| new | 2258 | PU.1 (9G7) Rabbit mAb | W, IP, IHC-P, IF-IC, F | H, M, (Mk) | mesoderm | hematopoietic stem cell | |
| new | 2216 | PU.1 (9G7) Rabbit mAb (Alexa Fluor® 488 Conjugate) | F | H, M | mesoderm | hematopoietic stem cell | |
| new | 2240 | PU.1 (9G7) Rabbit mAb (Alexa Fluor® 647 Conjugate) | F | H, M | mesoderm | hematopoietic stem cell | |
| new | 4119 | TWIST1 Antibody | W, IP | H, Mk | neural crest stem cell | Basic helix-loop-helix transcription factor that functions as a master regulator of embryonic morphogenesis. Plays essential roles in mesenchymal differentiation and osteogenic determination. Also plays critical role in neural crest formation and is a marker for neural crest stem cells. Upregulated in various human tumors and may play a role in EMT and metastasis. |
