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Western blot analysis of extracts from 293 and C6 cells, using S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody # 2217 (left) or the same antibody preincubated with S6 Ribosomal Protein Blocking Peptide (right).

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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using S6 Ribosomal Protein (5G10) Rabbit mAb #2217 preincubated with an irrelevant control pepetide (left), or with S6 Ribosomal Protein Blocking Peptide (right).

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Product Description

This peptide is used to block S6 Ribosomal Protein (5G10) Rabbit mAb # 2217 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody # 2217 signal completely in both immunohistochemistry and Western blotting.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting and immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room

temperature for 30 minutes before allowing to react with the blot.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).


No product specific background information is currently available for this product.


1.  Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.

2.  Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.

3.  Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.

4.  Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.

5.  Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.


Entrez-Gene Id 6194
Swiss-Prot Acc. P62753


For Research Use Only. Not For Use In Diagnostic Procedures.
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