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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb 9709 1 x 100 µl
H M 27 Rabbit IgG
HSP27 (G31) Mouse mAb 2402 1 x 100 µl
H Mk 27 Mouse IgG1

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).


1.  Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.

2.  Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.

3.  Rouse, J. et al. (1994) Cell 78, 1027-1037.

4.  Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.

5.  Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.

6.  Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.


Entrez-Gene Id 3315
Swiss-Prot Acc. P04792


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.