Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of c-Fos protein only when phosphorylated at Ser32. The antibody does not cross-react with other Fos proteins, including FosB, FRA1, and FRA2.


Species predicted to react based on 100% sequence homology: Hamster, Monkey, Bovine, Pig, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32 of human c-Fos protein.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb #5348.


The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).


1.  Tulchinsky, E. (2000) Histol Histopathol 15, 921-8.

2.  Dobrazanski, P. et al. (1991) Mol Cell Biol 11, 5470-8.

3.  Nakabeppu, Y. and Nathans, D. (1991) Cell 64, 751-9.

4.  Rosenberger, S.F. et al. (1999) J Biol Chem 274, 1124-30.

5.  Sasaki, T. et al. (2006) Mol Cell 24, 63-75.

6.  Basbous, J. et al. (2007) Mol Cell Biol 27, 3936-50.

7.  Kovary, K. and Bravo, R. (1991) Mol Cell Biol 11, 2451-9.

8.  Kovary, K. and Bravo, R. (1992) Mol Cell Biol 12, 5015-23.


Entrez-Gene Id 2353
Swiss-Prot Acc. P01100

Protein Specific References

Koga K et al. (2009) Immunity 30, 372–83


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

11919
Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb (PE Conjugate)