Upstream / Downstream
Explore pathways related to this product.
CST Antibody Performance Guarantee
Find answers on our FAQs page.
PTM information and tools available.
SignalKine™ Human LIF Sandwich ELISA Kit #11928
This Product is Unavailable
Figure 1. Supernatants from PBMCs, untreated or treated with PHA (10 µg/ml), were removed after 1 and 5 d. hLIF levels in the supernatants were quantified using SignalKine™ Human LIF Sandwich ELISA Kit.Learn more about how we got this image
Typical Data. Represented here are hLIF Standard Curves, one diluted in SignalKine™ Sample Diluent S01, a second diluted in cell culture media (RPMI + 10% FBS). Although these are typical of the standard curves that will be generated using this kit, a new set of standards should be run for each new experiment.Learn more about how we got this image
Gallery: SignalKine™ Human LIF Sandwich ELISA Kit #11928
- Figure 1. Supernatants from PBMCs, untreated or treated with PHA (10 µg/ml), were removed after 1 and 5 d. hLIF levels in the supernatants were quantified using SignalKine™ Human LIF Sandwich ELISA Kit.
- Typical Data. Represented here are hLIF Standard Curves, one diluted in SignalKine™ Sample Diluent S01, a second diluted in cell culture media (RPMI + 10% FBS). Although these are typical of the standard curves that will be generated using this kit, a new set of standards should be run for each new experiment.
|Product Includes||Volume||Solution Color|
|hLIF Rabbit mAb Coated Microwells||96 tests|
|Human LIF Standard (Lyophilized)||1 vial|
|SignalKine™ Assay Diluent A01||1.5 ml||Colorless|
|SignalKine™ Sample Diluent S01||25 ml||Brown|
|hLIF Detection Rabbit mAb (Biotinylated)(Lyophilized) 100X||1 vial|
|Detection Antibody Diluent||6 ml||Green|
|HRP-linked Streptavidin (Lyophilized) 100X||1 vial|
|HRP Diluent||6 ml||Red|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|TMB Substrate 7004||6 ml||Colorless|
|STOP Solution 7002||6 ml||Colorless|
|Sealing Tape||2 sheets|
SignalKine™ Human LIF Sandwich ELISA Kit from Cell Signaling Technology (CST™) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human LIF (hLIF) in multiple matrices. Unknown samples being tested for hLIF and hLIF Standards are added to low volume microwells, where the hLIF is captured by the coated hLIF Rabbit mAb. Following a washing step, a biotinylated hLIF Rabbit mAb is added to detect the captured hLIF. HRP-linked Streptavidin is then used for detection of the biotinylated hLIF Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hLIF in the sample.
SignalKine™ Human LIF Sandwich ELISA Kit detects hLIF in multiple matrices that be quantified by generating a standard curve with the recombinant hLIF protein standard provided. The hLIF standard range is from 31.25 to 2000 pg/ml. Samples containing higher levels of hLIF can be diluted to fit into the standard range.
Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise-induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.