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REACTIVITY
H
Product Includes Volume Solution Color
hLIF Rabbit mAb Coated Microwells 96 tests
Human LIF Standard (Lyophilized) 1 vial
SignalKine™ Assay Diluent A01 1.5 ml Colorless
SignalKine™ Sample Diluent S01 25 ml Brown
hLIF Detection Rabbit mAb (Biotinylated)(Lyophilized) 100X 1 vial
Detection Antibody Diluent 6 ml Green
HRP-linked Streptavidin (Lyophilized) 100X 1 vial
HRP Diluent 6 ml Red
ELISA Wash Buffer (20X) 25 ml Colorless
TMB Substrate 7004 6 ml Colorless
STOP Solution 7002 6 ml Colorless
Sealing Tape 2 sheets

Order Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Product Description

SignalKine™ Human LIF Sandwich ELISA Kit from Cell Signaling Technology (CST™) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human LIF (hLIF) in multiple matrices. Unknown samples being tested for hLIF and hLIF Standards are added to low volume microwells, where the hLIF is captured by the coated hLIF Rabbit mAb. Following a washing step, a biotinylated hLIF Rabbit mAb is added to detect the captured hLIF. HRP-linked Streptavidin is then used for detection of the biotinylated hLIF Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hLIF in the sample.

SignalKine™ Human LIF Sandwich ELISA Kit detects hLIF in multiple matrices that be quantified by generating a standard curve with the recombinant hLIF protein standard provided. The hLIF standard range is from 31.25 to 2000 pg/ml. Samples containing higher levels of hLIF can be diluted to fit into the standard range.


Specificity / Sensitivity


Species Reactivity: Human

Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise-induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).


1.  Mathieu, M.E. et al. (2012) Stem Cell Rev 8, 1-15.

2.  Broholm, C. and Pedersen, B.K. (2010) Exerc Immunol Rev 16, 77-85.

3.  Cao, W. et al. (2011) Immunity 35, 273-84.

4.  Ullah, U. et al. (2012) Sci Rep 2, 464.


Entrez-Gene Id 3976
Swiss-Prot Acc. P15018


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.