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To Purchase # 11946SC

11946SC 10 µg (With Carrier) $159.00
11946SF 10 µg (Carrier Free) $159.00
11946LC 50 µg (With Carrier) $459.00
11946LF 50 µg (Carrier Free) $459.00
$ 0. 00

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Source / Purification

Recombinant Human TL1A Leu72-Leu251 (Accession #NP_095150) was expressed in E.coli at Cell Signaling Technology.

Product Description

Purity:

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hTL1A. All lots are greater than 95% pure.


Molecular Formula:

Recombinant hTL1A has a calculated MW of 20,0473. DTT-reduced protein migrates as a 21 kDa polypeptide. The nonreduced protein migrates as a 21 kDa monomer and 38 kDa cystine-linked homodimer. The expected amino terminus of recombinant hTL1A was verified by amino acid sequencing.


Bioactivity:

The bioactivity of hTL1A was determined in a TF-1 cell viability assay. The ED50 of each lot is between 2-15 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hTL1A.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hTL1A. Carrier free: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

TL1A (TNFSF15), a member of the TNF superfamily of proteins, is a splice variant of the TL1/VEGI gene (1). Endothelial cells, monocytes, macrophages, and dendritic cells express TL1A, which is upregulated by proinflammatory cytokines, microorganisms, and AMPK activation (1-4). TL1A activates the NF-κB and JNK pathways through its receptor, DR3 (1,5). TL1A may function as a costimulatory signal for T cell activation, specifically regulating Th17 cell development and proliferation (1,2,6). Mouse models suggest a role for TL1A as a driver for the inflammation and pathogenesis associated with inflammatory bowel disease (7,8).


1.  Migone, T.S. et al. (2002) Immunity 16, 479-92.

2.  Jones, G.W. et al. (2011) FASEB J 25, 409-19.

3.  Zhou, J. et al. (2011) Oncogene 30, 1892-900.

4.  Shih, D.Q. et al. (2009) Eur J Immunol 39, 3239-50.

5.  Haridas, V. et al. (1999) Oncogene 18, 6496-504.

6.  Pappu, B.P. et al. (2008) J Exp Med 205, 1049-62.

7.  Meylan, F. et al. (2011) Mucosal Immunol 4, 172-85.

8.  Taraban, V.Y. et al. (2011) Mucosal Immunol 4, 186-96.


Entrez-Gene Id 9966
Swiss-Prot Acc. O95150


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.