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130-140 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

M-RIP Antibody recognizes endogenous levels of total M-RIP protein.

Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine, Dog

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro62 of human M-RIP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics (1-3). M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA (1-3). Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility (4,5). M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin (2,6). Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics (7,8).

1.  Surks, H.K. et al. (2003) J Biol Chem 278, 51484-93.

2.  Mulder, J. et al. (2004) Mol Biol Cell 15, 5516-27.

3.  Gebbink, M.F. et al. (1997) J Cell Biol 137, 1603-13.

4.  Kimura, K. et al. (1996) Science 273, 245-8.

5.  Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

6.  Riddick, N. et al. (2008) J Cell Biochem 103, 1158-70.

7.  Surks, H.K. et al. (2005) J Biol Chem 280, 42543-51.

8.  Koga, Y. and Ikebe, M. (2005) J Biol Chem 280, 4983-91.

Entrez-Gene Id 23164
Swiss-Prot Acc. Q6WCQ1

For Research Use Only. Not For Use In Diagnostic Procedures.
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M-RIP Antibody