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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb 5625 1 x 100 µl
H Mk 89 Rabbit IgG
PARP (46D11) Rabbit mAb 9532 1 x 100 µl
H M R Mk 116, 89 Rabbit 

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).


1.  Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.

2.  Nicholson, D. W. et al. (1995) Nature 376, 37-43.

3.  Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.

4.  Lazebnik, Y.A. et al. (1994) Nature 371, 346-7.

5.  Tewari, M. et al. (1995) Cell 81, 801-9.

6.  Oliver, F.J. et al. (1998) J Biol Chem 273, 33533-9.


Entrez-Gene Id 142
Swiss-Prot Acc. P09874


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.