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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb 5587 1 x 100 µl
H 66 Rabbit IgG
Estrogen Receptor α (D8H8) Rabbit mAb 8644 1 x 100 µl
H 66 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).


1.  Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.

2.  Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41.

3.  Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15.

4.  Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24.

5.  Chen, D. et al. (2000) Mol Cell 6, 127-37.

6.  Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84.


Entrez-Gene Id 2099
Swiss-Prot Acc. P03372


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.