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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-PERK (Thr980) (16F8) Rabbit mAb 3179 100 µl
Western Blotting
R 170 Rabbit IgG
PERK (C33E10) Rabbit mAb 3192 100 µl
Western Blotting
H M R Mk 140 Rabbit IgG

Product Usage Information


Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

1.  Harding, H. et al. (2000) Mol. Cell 5, 897-904.

2.  Harding, H. et al. (2000) Mol. Cell 5, 897-904.

3.  Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

4.  Harding, H.P. et al. (1999) Nature 397, 271-4.

Entrez-Gene Id 9451 , 13666
Swiss-Prot Acc. Q9NZJ5 , Q9Z2B5

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.