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REACTIVITY SENSITIVITY MW (kDa) Isotype
57-65 Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

c-Myc (D84C12) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Cell Signaling Technology cat. #2276, #3739, or #2278).


Species predicted to react based on 100% sequence homology: Monkey, Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of c-Myc.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated c-Myc (D84C12) XP® Rabbit mAb #5605.


Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).


1.  Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.

2.  Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.

3.  Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.

4.  Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.


Entrez-Gene Id 4609
Swiss-Prot Acc. P01106


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.