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REACTIVITY SENSITIVITY MW (kDa) Isotype
M Rabbit IgG

The ability of Mouse IL-3 Neutralizing (D6C1) Rabbit mAb to inhibit mIL-3-induced BaF3 cell proliferation was assessed. Cells were incubated with increasing concentrations of antibody in the presence of mIL-3 #8923 (250 pg/ml). After 72 hr, viable cells were detected by incubation with a tetrazolium salt and the OD450 was determined.

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The proliferation of BaF3 cells treated with increasing amounts of mIL-3 #8923 was determined. After a 72 hr treatment, cells were incubated with a tetrazolium salt and the OD450 was determined.

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Product Usage Information

CST recommends incubation of the neutralizing antibody with the intended target for 1 hr at 37ºC before addition to the experiment at an optimal concentration determined by the user.


Application Dilutions
Neutralizing 1:1

Formulation:

Lyophilized from a 0.2 µm filtered solution in 10 mM HEPES with trehalose.


Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Mouse IL-3 Neutralizing (D6C1) Rabbit mAb binds to mouse IL-3 and neutralizes its effects in a BaF3 cell proliferation assay. This antibody does not cross-react with human IL-3, human IL-5, human GM-CSF, or mouse GM-CSF.


Species Reactivity: Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant mouse IL-3 protein.

Product Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse IL-3 Neutralizing (D6C1) Rabbit mAb has been shown to neutralize the proliferation of IL-3-dependent BaF3 cells in vitro with an ND50 in the range of 0.5-3.5 µg/ml.


Endotoxin:

<0.1 EU/µg of antibody


IL-3 is produced by T cells, mast cells, and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid, and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support the culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3-specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2 and the PI3K/Akt pathway, and phosphorylation of Stat1/3/5/6 (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3-5).


1.  Reddy, E.P. et al. (2000) Oncogene 19, 2532-47.

2.  Hara, T. and Miyajima, A. (1992) EMBO J 11, 1875-84.

3.  Asquith, K.L. et al. (2008) J Immunol 180, 1199-206.

4.  Schroeder, J.T. et al. (2009) J Immunol 182, 2432-8.

5.  Munitz, A. et al. (2006) J Immunol 177, 77-83.


Entrez-Gene Id 3562
Swiss-Prot Acc. P08700


For Research Use Only. Not For Use In Diagnostic Procedures.
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