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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

FLI1 Antibody recognizes endogenous levels of total FLI1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of human FLI1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the Fli1 locus is a common retroviral insertion site for the Friend murine leukemia virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing's sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing’s sarcoma, an aggressive bone and soft tissue tumor (8,9).

1.  Spyropoulos, D.D. et al. (2000) Mol Cell Biol 20, 5643-52.

2.  Pimanda, J.E. et al. (2007) Proc Natl Acad Sci U S A 104, 17692-7.

3.  Jagadeeswaran, P. et al. (2010) Blood Cells Mol Dis 44, 175-80.

4.  Tijssen, M.R. et al. (2011) Dev Cell 20, 597-609.

5.  Ben-David, Y. et al. (1990) Proc Natl Acad Sci U S A 87, 1332-6.

6.  Kornblau, S.M. et al. (2011) Blood 118, 5604-12.

7.  May, W.A. et al. (1993) Proc Natl Acad Sci U S A 90, 5752-6.

8.  Braun, B.S. et al. (1995) Mol Cell Biol 15, 4623-30.

9.  Riggi, N. and Stamenkovic, I. (2007) Cancer Lett 254, 1-10.

Entrez-Gene Id 2313
Swiss-Prot Acc. Q01543

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FLI1 Antibody