Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To Purchase # 12296SC

12296SC 10 µg (With Carrier) $159.00
12296SF 10 µg (Carrier Free) $159.00
12296LC 50 µg (With Carrier) $459.00
12296LF 50 µg (Carrier Free) $459.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Source / Purification

Recombinant human FGF9 (hFGF9) Leu4-Ser208 (Accession #NP_31371) was expressed in E.coli at Cell Signaling Technology.

Product Description

Purity:

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hFGF9. All lots are greater than 95% pure.


Molecular Formula:

Recombinant hFGF9 has a calculated MW of 23,141 Da. DTT-reduced and nonreduced protein migrates as a 23 kDa polypeptide. The expected amino terminus of recombinant hFGF9 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of hFGF9 was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05-0.80 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hFGF9.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of hFGF9 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hFGF9. Carrier free: Lyophilized from a 0.22 μm filtered solution of hFGF9 in 20 mM Tris, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Fibroblast growth factor (FGF) 9 is a member of the larger FGF family of proteins that play key roles in development, cancer, and metabolism. Binding of FGF9 to its receptor requires interaction with heparin and induces receptor dimerization, subsequent transphosphorylation, and downstream activation of Erk, Akt, and PLCγ pathways (1). FGF9 is important for organ development and bone repair (2-4).


1.  Itoh, N. and Ornitz, D.M. (2011) J Biochem 149, 121-30.

2.  Yin, Y. et al. (2011) Development 138, 3169-77.

3.  Barak, H. et al. (2012) Dev Cell 22, 1191-207.

4.  Behr, B. et al. (2010) Proc Natl Acad Sci U S A 107, 11853-8.


Entrez-Gene Id 2254
Swiss-Prot Acc. P31371


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.