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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb 2197 1 x 100 µl
H 62 Rabbit IgG
Chk2 (D9C6) XP® Rabbit mAb 6334 1 x 100 µl
H 62 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).


1.  Ahn JY et al. (2000) Cancer Res 60, 5934–6

2.  Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.

3.  Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.

4.  Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.

5.  Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.

6.  Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.

7.  Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.

8.  Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.


Entrez-Gene Id 11200
Swiss-Prot Acc. O96017

Protein Specific References

Ahn JY et al. (2000) Cancer Res 60, 5934–6

Ahn J and Prives C (2002) J Biol Chem 277, 48418–26

Xu X et al. (2002) Mol Cell Biol 22, 4419–32

Lou Z et al. (2003) Nature 421, 957–61

Tsvetkov L et al. (2003) J Biol Chem 278, 8468–75

Bartkova J et al. (2004) Oncogene 23, 8545–51

Yin MB et al. (2004) Mol Pharmacol 66, 153–60

Li J and Stern DF (2005) J Biol Chem 280, 12041–50

Buscemi G et al. (2006) Mol Cell Biol 26, 7832–45

Yoda A et al. (2006) J Biol Chem 281, 24847–62

Sodha N et al. (2006) Cancer Res 66, 8966–70

Kass EM et al. (2007) J Biol Chem 282, 30311–21

Oliva-Trastoy M et al. (2007) Oncogene 26, 1449–58

Gabant G et al. (2008) J Mol Biol 380, 489–503

Guo X et al. (2010) J Biol Chem 285, 33348–57

Ahn JY et al. (2000) Cancer Res 60, 5934–6

Ahn J and Prives C (2002) J Biol Chem 277, 48418–26

Xu X et al. (2002) Mol Cell Biol 22, 4419–32

Lou Z et al. (2003) Nature 421, 957–61

Tsvetkov L et al. (2003) J Biol Chem 278, 8468–75

Bartkova J et al. (2004) Oncogene 23, 8545–51

Yin MB et al. (2004) Mol Pharmacol 66, 153–60

Li J and Stern DF (2005) J Biol Chem 280, 12041–50

Buscemi G et al. (2006) Mol Cell Biol 26, 7832–45

Yoda A et al. (2006) J Biol Chem 281, 24847–62

Sodha N et al. (2006) Cancer Res 66, 8966–70

Kass EM et al. (2007) J Biol Chem 282, 30311–21

Oliva-Trastoy M et al. (2007) Oncogene 26, 1449–58

Gabant G et al. (2008) J Mol Biol 380, 489–503

Guo X et al. (2010) J Biol Chem 285, 33348–57


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.