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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MyoD1 Antibody recognizes endogenous levels of total MyoD1 protein. This antibody also detects a 142 kDa protein of unknown origin.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly228 of human MyoD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Myoblast determination protein 1 (MyoD1), also called myogenic factor 3 (Myf3), is a member of the MyoD family of muscle specific bHLH transcription factors (1). This family is responsible for controlling specification of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types (2,3). Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins (4-6). Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways (5-7).

1.  Berkes, C.A. and Tapscott, S.J. (2005) Semin Cell Dev Biol 16, 585-95.

2.  Tapscott, S.J. (2005) Development 132, 2685-95.

3.  Davis, R.L. et al. (1987) Cell 51, 987-1000.

4.  de la Serna, I.L. et al. (2001) Nat Genet 27, 187-90.

5.  Aziz, A. et al. (2010) Epigenetics 5, 691-5.

6.  Guttridge, D.C. (2004) Curr Opin Clin Nutr Metab Care 7, 443-50.

7.  Ge, Y. and Chen, J. (2012) J Biol Chem 287, 43928-35.

Entrez-Gene Id 4654
Swiss-Prot Acc. P15172

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MyoD1 Antibody