Upstream / Downstream


Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee


To Purchase # 12680S

12680S 300 µl (3 nmol) $249.00
$ 0. 00

To get local purchase information on this product, click here


Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.



Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® γ-Catenin siRNA I (Mouse Specific) #12523 (+) or SignalSilence® γ-Catenin siRNA II (Mouse Specific) (+), using γ-Catenin Antibody #2309 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The γ-Catenin Antibody confirms silencing of γ-catenin expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Learn more about how we get our images

Product Usage Information

CST recommends transfection with 100 nM γ-Catenin siRNA II (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® γ-Catenin siRNA II (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit γ-catenin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the Drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9).

1.  Zhurinsky, J. et al. (2000) J. Cell Sci. 113 ( Pt 18), 3127-3139.

2.  Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.

3.  Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.

4.  Zhurinsky, J. et al. (2000) Mol. Cell Biol. 20, 4238-4252.

5.  Charpentier, E. et al. (2000) J. Cell Biol. 149, 503-520.

6.  Kolligs, F.T. et al. (2000) Genes Dev. 14, 1319-13131.

7.  Miravet, S. et al. (2003) Mol. Cell Biol. 23, 7391-7402.

8.  Hu, P. et al. (2006) J. Biol. Chem. 281, 12786-12791.

9.  Yin, T. et al. (2005) J. Biol. Chem. 280, 40355-40363.

Entrez-Gene Id 16480
Swiss-Prot Acc. Q02257

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.