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REACTIVITY SENSITIVITY MW (kDa) Isotype
H Rabbit IgG

The ability of Human IL-17A Neutralizing (D13C2) Rabbit mAb to inhibit IL-17A-induced IL-6 production was assessed. Human dermal fibroblasts were incubated with increasing concentrations of antibody in the presence of Human Interleukin-17A (hIL-17A) #8928 (15 ng/ml). After 48 hr, culture supernatants were harvested and assayed for IL-6 by ELISA and the OD450 was determined.

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The production of IL-6 by human dermal fibroblasts cultured with increasing concentrations of Human IL-17A (hIL-17A) #8928 was assessed. Media from cells incubated with IL-17A for 48 hours was collected and assayed for IL-6 by ELISA and the OD450 was determined.

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Product Usage Information

CST recommends incubation of the neutralizing antibody with the intended target for 1 hr at 37ºC before addition to the experiment at an optimal concentration determined by the user.


Application Dilutions
Neutralizing 1:1

Formulation:

Lyophilized from a 0.2 µm filtered solution in 10 mM HEPES with trehalose.


Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Human IL-17A Neutralizing (D13C2) Rabbit mAb binds to human IL-17A (hIL-17A) and neutralizes its ability to induce IL-6 production in human dermal fibroblast cells. This antibody shows 50% cross-reactivity with human IL-17A/F. This antibody does not cross-react with mouse IL-17A, human IL-25 or mouse IL-25 .


Species Reactivity: Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human IL-17A protein.

Product Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human IL-17A Neutralizing (D13C2) Rabbit mAb has been shown to neutralize the production of IL-6 from human dermal fibroblast cells in vitro with an ND50 in the range of 8-50 ng/ml.


Endotoxin:

Less than 0.1 EU/µg of antibody


IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6).


1.  Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.

2.  Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.

3.  Dubin, P.J. and Kolls, J.K. (2008) Immunol Rev 226, 160-71.

4.  Zrioual, S. et al. (2009) J Immunol 182, 3112-20.

5.  Wright, J.F. et al. (2008) J Immunol 181, 2799-805.

6.  Rahman, M.S. et al. (2006) J Immunol 177, 4064-71.


Entrez-Gene Id 3605
Swiss-Prot Acc. Q16552


For Research Use Only. Not For Use In Diagnostic Procedures.
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