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Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949
Gallery: Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949
Color-coded Prestained Protein Marker, High Range (43-315 kDa) is a mixture of purified proteins, covalently coupled to blue, green, orange, or pink dyes, that resolves to 8 bands between 43 and 315 kDa when electrophoresed. The protein concentrations are carefully balanced for even intensity. The covalent coupling of dye to protein affects the electrophoretic mobility in SDS-PAGE gels relative to uncoupled proteins. The apparent molecular weights of the prestained proteins are shown in the image to the right.Bioactivity:
Storage: Supplied in 62.5 mM Tris-H3PO4 (pH 7.5 at 25ºC), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol. Store at –20°C for up to one year.
Important: Do Not Boil Protein Marker
1. Thaw the protein ladder at room temperature.
2. Gently vortex solution to ensure the mixture is homogeneous.
3. Load the appropriate volume of the protein marker per lane, as specified below:
0.75-1.0 mm thick: load 10 μl
1.5 mm thick: load 20 μl
0.75-1.0 mm thick: load 20 μl
1.5 mm thick: load 40 μl
4. Unused ladder may be returned to -20ºC for long-term storage.Storage: Store at -20°C.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.