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55 Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Atg5 (D6M7Q) Rabbit mAb recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12. This antibody may cross-react with a protein of unknown origin at ~80 kDa in some cell lines.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly232 of human Atg5 protein.

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

1.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.

2.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.

3.  Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.

4.  Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.

5.  Mizushima, N. et al. (1998) Nature 395, 395-8.

6.  Suzuki, K. et al. (2001) EMBO J 20, 5971-81.

7.  Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.

8.  Shintani, T. et al. (1999) EMBO J 18, 5234-41.

Entrez-Gene Id 9474
Swiss-Prot Acc. Q9H1Y0

For Research Use Only. Not For Use In Diagnostic Procedures.
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Atg5 (D6M7Q) Rabbit mAb