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To Purchase # 13173S

13173S 100 µl (50 tests) $329.00
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REACTIVITY SENSITIVITY Isotype
H M R Endogenous Rabbit IgG

Confocal immunofluorescent analysis of HeLa cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (4 hr, middle), or untreated (right) using LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #13173 (red) and β-Actin (8H10D10) Mouse mAb #3700 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Usage Information

Application Dilutions
Immunofluorescence 1:50

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total LC3A and LC3B proteins.


Species Reactivity: Human, Mouse, Rat
Species predicted to react based on 100% sequence homology: Monkey, Xenopus, Bovine, Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu44 of human LC3B protein (conserved in LC3A).

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated LC3A/B (D3U4C) XP® Rabbit mAb #12741.


Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).


1.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.

2.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.

3.  Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.

4.  Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.

5.  Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.

6.  Lang, T. et al. (1998) EMBO J. 17, 3597-607.

7.  Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.

8.  He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.

9.  Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.

10.  Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.

11.  Ichimura, Y. et al. (2000) Nature 408, 488-92.


Entrez-Gene Id 84557, 81631
Swiss-Prot Acc. Q9H492, Q9GZQ8


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Life Technologies Corporation.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.