Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To Purchase # 13348S

13348S 300 µl (3 nmol) $249.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY
H

Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® PTP1B siRNA I (+) or SignalSilence® PTP1B siRNA II #13380 (+), using PTP1B Antibody #5311 (upper) or β-Actin (D8A6) Rabbit mAb #8457 (lower). The PTP1B Antibody confirms silencing of PTP1B expression, while the β-Actin (D8A6) Rabbit mAb is used as a loading control.

Learn more about how we got this image
Image

Product Usage Information

CST recommends transfection with 100 nM SignalSilence® PTP1B siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® PTP1B siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PTP1B expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Protein-tyrosine phosphatase 1B (PTP1B) is the original member of the protein tyrosine phosphatase (PTP) family of proteins (1). PTP1B is highly expressed in insulin-responsive tissues and negatively regulates insulin, as well as integrin, through dephosphorylation of phosphotyrosine residues (2-4). PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes (5).


1.  Liang, F. et al. (2005) J Biol Chem 280, 24857-63.

2.  Kenner, K.A. et al. (1996) J Biol Chem 271, 19810-6.

3.  Klaman, L.D. et al. (2000) Mol Cell Biol 20, 5479-89.

4.  Liu, F. et al. (1998) Curr Biol 8, 173-6.

5.  Elchebly, M. et al. (1999) Science 283, 1544-8.


Entrez-Gene Id 5770
Swiss-Prot Acc. P18031


For Research Use Only. Not For Use In Diagnostic Procedures.
CST™ is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.